This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3pf2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:30, 1 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==The Crystal Structure of the Major Pilin GBS80 of Streptococcus agalactiae 35kDa C-terminal fragment==
==The Crystal Structure of the Major Pilin GBS80 of Streptococcus agalactiae 35kDa C-terminal fragment==
-
<StructureSection load='3pf2' size='340' side='right' caption='[[3pf2]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+
<StructureSection load='3pf2' size='340' side='right'caption='[[3pf2]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3pf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF2 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3pf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF2 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pg2|3pg2]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GBS80, SAG0645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae serogroup V])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf2 OCA], [https://pdbe.org/3pf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf2 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf2 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf2 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf2 PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q8E0S9_STRA5 Q8E0S9_STRA5]
-
The Gram-positive pathogen Streptococcus agalactiae, known as group B Streptococcus (GBS), is the leading cause of bacterial septicemia, pneumonia, and meningitis among neonates. GBS assembles two types of pili-pilus islands (PIs) 1 and 2-on its surface to adhere to host cells and to initiate colonization for pathogenesis. The GBS PI-1 pilus is made of one major pilin, GBS80, which forms the pilus shaft, and two secondary pilins, GBS104 and GBS52, which are incorporated into the pilus at various places. We report here the crystal structure of the 35-kDa C-terminal fragment from GBS80, which is composed of two IgG-like domains (N2-N3). The structure was solved by single-wavelength anomalous dispersion using sodium-iodide-soaked crystals and diffraction data collected at the home source. The N2 domain exhibits a cnaA/DEv-IgG fold with two calcium-binding sites, while the N3 domain displays a cnaB/IgG-rev fold. We have built a model for full-length GBS80 (N1, N2, and N3) with the help of available homologous major pilin structures, and we propose a model for the GBS PI-1 pilus shaft. The N2 and N3 domains are arranged in tandem along the pilus shaft, whereas the respective N1 domain is tilted by approximately 20 degrees away from the pilus axis. We have also identified a pilin-like motif in the minor pilin GBS52, which might aid its incorporation at the pilus base.
+
-
 
+
-
A Model for Group B Streptococcus Pilus Type 1: The Structure of a 35-kDa C-Terminal Fragment of the Major Pilin GBS80.,Vengadesan K, Ma X, Dwivedi P, Ton-That H, Narayana SV J Mol Biol. 2011 Apr 15;407(5):731-43. Epub 2011 Feb 17. PMID:21333654<ref>PMID:21333654</ref>
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
==See Also==
-
</div>
+
*[[Pilin 3D structures|Pilin 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Streptococcus agalactiae serogroup v]]
+
[[Category: Large Structures]]
-
[[Category: Narayana, S V.L]]
+
[[Category: Streptococcus agalactiae serogroup V]]
-
[[Category: Vengadesan, K]]
+
[[Category: Narayana SVL]]
-
[[Category: Backbone pilin]]
+
[[Category: Vengadesan K]]
-
[[Category: Cell adhesion]]
+
-
[[Category: Cna a type]]
+
-
[[Category: Cna b type]]
+
-
[[Category: Gbs80]]
+
-
[[Category: Gram-positive bacterial cell surface]]
+
-
[[Category: Ig-like fold]]
+
-
[[Category: Igg-dev fold]]
+
-
[[Category: Igg-rev fold]]
+
-
[[Category: Isopeptide bond formation]]
+
-
[[Category: Major pilin]]
+
-
[[Category: Major pilin gbs80]]
+
-
[[Category: Pilus shaft component]]
+
-
[[Category: Pilus subunit]]
+

Current revision

The Crystal Structure of the Major Pilin GBS80 of Streptococcus agalactiae 35kDa C-terminal fragment

PDB ID 3pf2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3pf2"
Personal tools