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3ou6

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==DhpI-SAM complex==
==DhpI-SAM complex==
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<StructureSection load='3ou6' size='340' side='right' caption='[[3ou6]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3ou6' size='340' side='right'caption='[[3ou6]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ou6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_luridus Streptomyces luridus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OU6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ou6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_luridus Streptomyces luridus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OU6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ou2|3ou2]], [[3ou7|3ou7]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dhpI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=67320 Streptomyces luridus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ou6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ou6 OCA], [https://pdbe.org/3ou6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ou6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ou6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ou6 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ou6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ou6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ou6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ou6 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/D7PC21_STRLR D7PC21_STRLR]
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Phosphonate natural products possess a range of biological activities as a consequence of their ability to mimic phosphate esters or tetrahedral intermediates formed in enzymatic reactions involved in carboxyl group metabolism. The dianionic form of these compounds at pH 7 poses a drawback with respect to their ability to mimic carboxylates and tetrahedral intermediates. Microorganisms producing phosphonates have evolved two solutions to overcome this hurdle: biosynthesis of monoanionic phosphinates containing two P-C bonds or esterification of the phosphonate group. The latter solution was first discovered for the antibiotic dehydrophos that contains a methyl ester of a phosphonodehydroalanine group. We report here the expression, purification, substrate scope, and structure of the O-methyltransferase from the dehydrophos biosynthetic gene cluster. The enzyme utilizes S-adenosylmethionine to methylate a variety of phosphonates including 1-hydroxyethylphosphonate, 1,2-dihydroxyethylphosphonate, and acetyl-1-aminoethylphosphonate. Kinetic analysis showed that the best substrates are tripeptides containing as C-terminal residue a phosphonate analog of alanine suggesting the enzyme acts late in the biosynthesis of dehydrophos. These conclusions are corroborated by the X-ray structure that reveals an active site that can accommodate a tripeptide substrate. Furthermore, the structural studies demonstrate a conformational change brought about by substrate or product binding. Interestingly, the enzyme has low substrate specificity and was used to methylate the clinical antibiotic fosfomycin and the antimalaria clinical candidate fosmidomycin, showing its promise for applications in bioengineering.
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Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis.,Lee JH, Bae B, Kuemin M, Circello BT, Metcalf WW, Nair SK, van der Donk WA Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17557-62. Epub 2010 Sep 27. PMID:20876132<ref>PMID:20876132</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
*[[SAM-dependent methyltransferase|SAM-dependent methyltransferase]]
*[[SAM-dependent methyltransferase|SAM-dependent methyltransferase]]
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== References ==
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*[[SAM-dependent methyltrasferase 3D structures|SAM-dependent methyltrasferase 3D structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces luridus]]
[[Category: Streptomyces luridus]]
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[[Category: Bae, B]]
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[[Category: Bae B]]
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[[Category: Nair, S K]]
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[[Category: Nair SK]]
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[[Category: O-methyltransferase]]
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[[Category: Sam]]
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[[Category: Transferase]]
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Current revision

DhpI-SAM complex

PDB ID 3ou6

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