3rle

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of GRASP55 GRASP domain (residues 7-208)

Structural highlights

3rle is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.649Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GORS2_HUMAN Plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.^[1] ^[2]

Publication Abstract from PubMed

Biogenesis of the ribbon-like membrane network of the mammalian Golgi requires membrane tethering by the conserved GRASP domain in GRASP65 and GRASP55, yet the tethering mechanism is not fully understood. Here, we report the crystal structure of the GRASP55 GRASP domain, which revealed an unusual arrangement of two tandem PDZ folds that more closely resemble prokaryotic PDZ domains. Biochemical and functional data indicated that the interaction between the ligand-binding pocket of PDZ1 and an internal ligand on PDZ2 mediates the GRASP self-interaction, and structural analyses suggest that this occurs via a unique mode of internal PDZ ligand recognition. Our data uncover the structural basis for ligand specificity and provide insight into the mechanism of GRASP-dependent membrane tethering of analogous Golgi cisternae.

Structure of the Membrane-tethering GRASP Domain Reveals a Unique PDZ Ligand Interaction That Mediates Golgi Biogenesis.,Truschel ST, Sengupta D, Foote A, Heroux A, Macbeth MR, Linstedt AD J Biol Chem. 2011 Jun 10;286(23):20125-9. Epub 2011 Apr 22. PMID:21515684^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA. GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system. EMBO J. 1999 Sep 15;18(18):4949-60. PMID:10487747 doi:http://dx.doi.org/10.1093/emboj/18.18.4949
↑ Truschel ST, Sengupta D, Foote A, Heroux A, Macbeth MR, Linstedt AD. Structure of the Membrane-tethering GRASP Domain Reveals a Unique PDZ Ligand Interaction That Mediates Golgi Biogenesis. J Biol Chem. 2011 Jun 10;286(23):20125-9. Epub 2011 Apr 22. PMID:21515684 doi:10.1074/jbc.C111.245324
↑ Truschel ST, Sengupta D, Foote A, Heroux A, Macbeth MR, Linstedt AD. Structure of the Membrane-tethering GRASP Domain Reveals a Unique PDZ Ligand Interaction That Mediates Golgi Biogenesis. J Biol Chem. 2011 Jun 10;286(23):20125-9. Epub 2011 Apr 22. PMID:21515684 doi:10.1074/jbc.C111.245324

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rle"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of GRASP55 GRASP domain (residues 7-208)==
-<StructureSection load='3rle' size='340' side='right' caption='[[3rle]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='3rle' size='340' side='right'caption='[[3rle]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3rle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RLE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3rle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RLE FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.649&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GORASP2, GOLPH6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rle RCSB], [http://www.ebi.ac.uk/pdbsum/3rle PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rle OCA], [https://pdbe.org/3rle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rle RCSB], [https://www.ebi.ac.uk/pdbsum/3rle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rle ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GORS2_HUMAN GORS2_HUMAN] Plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.<ref>PMID:10487747</ref> <ref>PMID:21515684</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3rle" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 20: / Line 24: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Foote, A]]
+[[Category: Large Structures]]
-[[Category: Heroux, A]]
+[[Category: Foote A]]
-[[Category: Linstedt, A D]]
+[[Category: Heroux A]]
-[[Category: Macbeth, M R]]
+[[Category: Linstedt AD]]
-[[Category: Sengupta, D]]
+[[Category: Macbeth MR]]
-[[Category: Truschel, S T]]
+[[Category: Sengupta D]]
-[[Category: Golgi]]
+[[Category: Truschel ST]]
-[[Category: Golgin]]
-[[Category: Membrane protein]]
-[[Category: Pdz]]
-[[Category: Tether]]

3rle

From Proteopedia

Current revision

Crystal Structure of GRASP55 GRASP domain (residues 7-208)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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