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1qzn

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[[Image:1qzn.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus==
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|PDB= 1qzn |SIZE=350|CAPTION= <scene name='initialview01'>1qzn</scene>, resolution 1.90&Aring;
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<StructureSection load='1qzn' size='340' side='right'caption='[[1qzn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1qzn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZN FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= ScaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35830 Acetivibrio cellulolyticus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzn OCA], [https://pdbe.org/1qzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzn RCSB], [https://www.ebi.ac.uk/pdbsum/1qzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzn ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus'''
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[https://www.uniprot.org/uniprot/Q7WYN3_9FIRM Q7WYN3_9FIRM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The incorporation of enzymes into the multi-enzyme cellulosome complex and its anchoring to the bacterial cell surface are dictated by a set of binding interactions between two complementary protein modules: the cohesin and the dockerin. In this work, the X-ray crystal structure of a type-II cohesin from scaffoldin A of Bacteroides cellulosolvens has been determined to a resolution of 1.6 angstroms using molecular replacement. The type-II B. cellulosolvens cohesin (Bc-cohesin-II) is the first detailed description of a crystal structure for a type-II cohesin, and its features were compared with the known type-I cohesins from Clostridium thermocellum and Clostridium cellulolyticum (Ct-cohesin-I and Cc-cohesin-I, respectively). The overall jelly-roll topology of the type-II Bc-cohesin is very similar to that observed for the type-I cohesins with three additional secondary structures: an alpha-helix and two "beta-flaps" that disrupt the normal course of a beta-strand. In addition, beta-strand 5 is elevated by approximately 4 angstroms on the surface of the molecule, relative to the type-I Ct and Cc-cohesins. Like its type-I analogue, the hydrophobic/aromatic core of Bc-cohesin-II comprises an upper and lower core, but an additional aromatic patch and conserved tryptophan at the crown of the molecule serves to stabilize the alpha-helix of the type-II cohesin. Comparison of Bc-cohesin-II with the known type-I cohesin-dockerin heterodimer suggests that each of the additional secondary structural elements assumes a flanking position relative to the putative dockerin-binding surface. The raised ridge formed by beta-strand 5 confers additional distinctive topographic features to the proposed binding interface that collectively distinguish between the type-II and type-I cohesins.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzn_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1QZN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZN OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzn ConSurf].
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Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements., Noach I, Frolow F, Jakoby H, Rosenheck S, Shimon LW, Lamed R, Bayer EA, J Mol Biol. 2005 Apr 22;348(1):1-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15808849 15808849]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Acetivibrio cellulolyticus]]
[[Category: Acetivibrio cellulolyticus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bayer, E A.]]
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[[Category: Bayer EA]]
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[[Category: Frolow, F.]]
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[[Category: Frolow F]]
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[[Category: Lamed, R.]]
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[[Category: Lamed R]]
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[[Category: Noach, I.]]
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[[Category: Noach I]]
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[[Category: Qi, X.]]
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[[Category: Qi X]]
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[[Category: Rosenheck, S.]]
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[[Category: Rosenheck S]]
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[[Category: Shimon, L J.W.]]
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[[Category: Shimon LJW]]
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[[Category: keywords: cohesins type ii; cellulosome;]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:44:59 2008''
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Current revision

Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus

PDB ID 1qzn

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