1r7s

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[[Image:1r7s.jpg|left|200px]]
 
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{{Structure
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==PUTIDAREDOXIN (Fe2S2 ferredoxin), C73G mutant==
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|PDB= 1r7s |SIZE=350|CAPTION= <scene name='initialview01'>1r7s</scene>, resolution 1.91&Aring;
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<StructureSection load='1r7s' size='340' side='right'caption='[[1r7s]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene>
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<table><tr><td colspan='2'>[[1r7s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R7S FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91&#8491;</td></tr>
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|GENE= CAMB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r7s OCA], [https://pdbe.org/1r7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r7s RCSB], [https://www.ebi.ac.uk/pdbsum/1r7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r7s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PUTX_PSEPU PUTX_PSEPU] The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r7/1r7s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r7s ConSurf].
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<div style="clear:both"></div>
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'''PUTIDAREDOXIN (Fe2S2 ferredoxin), C73G mutant'''
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==See Also==
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of the C73G mutant of putidaredoxin (Pdx), the Fe(2)S(2) ferredoxin that supplies electrons to cytochrome CYP101 (p450cam) for camphor oxidation, is reported at 1.9 A resolution in a C2 crystal form. The structure was solved by single-wavelength iron anomalous diffraction, which yielded electron density above the 2sigma level for over 97% of the non-H atoms in the protein. The final structure with R = 0.19 and R(free) = 0.21 has been deposited in the Protein Data Bank with accession code 1r7s. The C2 crystal contains three Pdx molecules in the asymmetric unit, giving three independent models of the protein that are very similar (r.m.s.d. &lt; 0.3 A for the 106 C(alpha) atoms). The unusually high solvent fraction of 80% results in comparatively few crystal-packing artifacts. The structure is briefly compared with the recently reported crystal structures of the C73S and C73S/C85S mutants. In general, the eight independent molecules in the three crystal structures (three in C73G, three in C73S and two in C73S/C85S) are much more similar to each other than to the previously reported NMR structure of wild-type Pdx in solution. The present findings show a unanimous structure in some regions crucial for electron-transfer interactions, including the cluster-binding loop 39-48 and the cytochrome-interaction region of Asp38 and Trp106. In addition, the Cys45 amide group donates a hydrogen bond to cluster sulfur S1, with Ala46 adopting an Lalpha conformation, in all three molecules in the crystal.
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[[Category: Large Structures]]
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==About this Structure==
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1R7S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7S OCA].
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==Reference==
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Structure of C73G putidaredoxin from Pseudomonas putida., Smith N, Mayhew M, Holden MJ, Kelly H, Robinson H, Heroux A, Vilker VL, Gallagher DT, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):816-22. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103126 15103126]
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[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Gallagher DT]]
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[[Category: Gallagher, D T.]]
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[[Category: Heroux A]]
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[[Category: Heroux, A.]]
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[[Category: Holden MJ]]
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[[Category: Holden, M J.]]
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[[Category: Kelly H]]
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[[Category: Kelly, H.]]
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[[Category: Mayhew M]]
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[[Category: Mayhew, M.]]
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[[Category: Robinson H]]
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[[Category: Robinson, H.]]
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[[Category: Smith N]]
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[[Category: Smith, N.]]
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[[Category: FES]]
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[[Category: cytochrome p450cam]]
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[[Category: electron transfer]]
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[[Category: ferredoxin]]
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[[Category: iron-sulfur cluster]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:58 2008''
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Current revision

PUTIDAREDOXIN (Fe2S2 ferredoxin), C73G mutant

PDB ID 1r7s

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