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1r9g

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[[Image:1r9g.jpg|left|200px]]
 
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{{Structure
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==Three-dimensional Structure of YaaE from Bacillus subtilis==
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|PDB= 1r9g |SIZE=350|CAPTION= <scene name='initialview01'>1r9g</scene>, resolution 2.5&Aring;
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<StructureSection load='1r9g' size='340' side='right'caption='[[1r9g]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1r9g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9G FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= YAAE, BSU00120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9g OCA], [https://pdbe.org/1r9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9g RCSB], [https://www.ebi.ac.uk/pdbsum/1r9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9g ProSAT]</span></td></tr>
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</table>
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'''Three-dimensional Structure of YaaE from Bacillus subtilis'''
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== Function ==
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[https://www.uniprot.org/uniprot/PDXT_BACSU PDXT_BACSU] Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to PdxS.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The structure of YaaE from Bacillus subtilis was determined at 2.5-A resolution. YaaE is a member of the triad glutamine aminotransferase family and functions in a recently identified alternate pathway for the biosynthesis of vitamin B(6). Proposed active residues include conserved Cys-79, His-170, and Glu-172. YaaE shows similarity to HisH, a glutaminase involved in histidine biosynthesis. YaaD associates with YaaE. A homology model of this protein was constructed. YaaD is predicted to be a (beta/alpha)(8) barrel on the basis of sequence comparisons. The predicted active site includes highly conserved residues 211-216 and 233-235. Finally, a homology model of a putative YaaD-YaaE complex was prepared using the structure of HisH-F as a model. This model predicts that the ammonia molecule generated by YaaE is channeled through the center of the YaaD barrel to the putative YaaD active site.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9g_consurf.spt"</scriptWhenChecked>
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1R9G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9G OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis., Bauer JA, Bennett EM, Begley TP, Ealick SE, J Biol Chem. 2004 Jan 23;279(4):2704-11. Epub 2003 Oct 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14585832 14585832]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r9g ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bauer, J A.]]
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[[Category: Bauer JA]]
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[[Category: Begley, T P.]]
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[[Category: Begley TP]]
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[[Category: Bennett, E M.]]
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[[Category: Bennett EM]]
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[[Category: Ealick, S E.]]
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[[Category: Ealick SE]]
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[[Category: alpha/beta protein]]
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[[Category: cofactor biosynthesis]]
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[[Category: glutaminase]]
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[[Category: triad amidotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:48:31 2008''
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Current revision

Three-dimensional Structure of YaaE from Bacillus subtilis

PDB ID 1r9g

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