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1rjv

From Proteopedia

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[[Image:1rjv.gif|left|200px]]
 
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{{Structure
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==Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy==
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|PDB= 1rjv |SIZE=350|CAPTION= <scene name='initialview01'>1rjv</scene>
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<StructureSection load='1rjv' size='340' side='right'caption='[[1rjv]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1rjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJV FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= PVALB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rjv OCA], [https://pdbe.org/1rjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rjv RCSB], [https://www.ebi.ac.uk/pdbsum/1rjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rjv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRVA_HUMAN PRVA_HUMAN] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/1rjv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rjv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
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'''Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy'''
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Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin.,Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A Biochemistry. 2004 May 11;43(18):5562-73. PMID:15122922<ref>PMID:15122922</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1rjv" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
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*[[Parvalbumin|Parvalbumin]]
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== References ==
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==About this Structure==
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<references/>
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1RJV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJV OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin., Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A, Biochemistry. 2004 May 11;43(18):5562-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15122922 15122922]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Baig, I.]]
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[[Category: Baig I]]
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[[Category: Bertini, I.]]
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[[Category: Bertini I]]
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[[Category: Bianco, C Del.]]
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[[Category: Del Bianco C]]
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[[Category: Gupta, Y K.]]
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[[Category: Gupta YK]]
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[[Category: Lee, Y M.]]
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[[Category: Lee YM]]
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[[Category: Luchinat, C.]]
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[[Category: Luchinat C]]
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[[Category: Quattrone, A.]]
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[[Category: Quattrone A]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: CA]]
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[[Category: calcium]]
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[[Category: ef-hand]]
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[[Category: lanthanide]]
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[[Category: nmr]]
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[[Category: parvalbumin]]
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[[Category: spine]]
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[[Category: structural genomic]]
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[[Category: structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:34 2008''
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Current revision

Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy

PDB ID 1rjv

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