3wcq

<StructureSection load='3wcq' size='340' side='right' caption='[[3wcq]], [[Resolution|resolution]] 0.97&Aring;' scene=''>

<table><tr><td colspan='2'>[[3wcq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae Cyanidioschyzon merolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WCQ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=45157 Cyanidioschyzon merolae])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wcq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wcq RCSB], [http://www.ebi.ac.uk/pdbsum/3wcq PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50 degrees C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97A) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c.

 

Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.,Ueno Y, Matsumoto T, Yamano A, Imai T, Morimoto Y Biochem Biophys Res Commun. 2013 Jul 12;436(4):736-9. doi:, 10.1016/j.bbrc.2013.06.029. Epub 2013 Jun 18. PMID:23792094<ref>PMID:23792094</ref>

 

*[[Ferredoxin|Ferredoxin]]

[[Category: Imai, T]]

[[Category: Matsumoto, T]]

[[Category: Morimoto, Y]]

[[Category: Ueno, Y]]

[[Category: Yamano, A]]

[[Category: Fnr]]