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| - | [[Image:2bpl.gif|left|200px]]<br /> | + | #REDIRECT [[4amv]] This PDB entry is obsolete and replaced by 4amv |
| - | <applet load="2bpl" size="450" color="white" frame="true" align="right" spinBox="true"
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| - | caption="2bpl, resolution 2.05Å" />
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| - | '''E.COLI GLUCOSAMINE-6P SYNTHASE IN COMPLEX WITH FRUCTOSE-6P'''<br />
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| - | ==Overview==
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| - | Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from, fructose-6P and glutamine and uses a channel to transfer ammonia from its, glutaminase to its synthase active site. X-ray structures of, glucosamine-6P synthase have been determined at 2.05 Angstroms resolution, in the presence of fructose-6P and at 2.35 Angstroms resolution in the, presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine, affinity analog that covalently modifies the N-terminal catalytic, cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate, formed during hydrolysis of glutamine. The fixation of the glutamine, analog activates the enzyme through several major structural changes: 1), the closure of a loop to shield the glutaminase site accompanied by, significant domain hinging, 2) the activation of catalytic residues, involved in glutamine hydrolysis, i.e. the alpha-amino group of Cys-1 and, Asn-98 that is positioned to form the oxyanion hole, and 3) a 75 degrees, rotation of the Trp-74 indole group that opens the ammonia channel.
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| - | ==About this Structure==
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| - | 2BPL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with F6R as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BPL OCA].
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| - | ==Reference==
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| - | Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16339762 16339762]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Glutamine--fructose-6-phosphate transaminase (isomerizing)]]
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| - | [[Category: Single protein]]
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| - | [[Category: Golinelli-Pimpaneau, B.]]
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| - | [[Category: Mouilleron, S.]]
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| - | [[Category: F6R]]
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| - | [[Category: amidotransferase]]
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| - | [[Category: ammonia channeling]]
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| - | [[Category: fructose 6-phosphate]]
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| - | [[Category: glucosamine 6-phosphate synthase]]
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| - | [[Category: glutamine amidotransferase]]
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| - | [[Category: n terminal nucleophile]]
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| - | [[Category: transferase]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:42:16 2007''
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