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4gk9

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==Crystal structure of Burkholderia oklahomensis agglutinin (BOA) bound to 3a,6a-mannopentaose==
==Crystal structure of Burkholderia oklahomensis agglutinin (BOA) bound to 3a,6a-mannopentaose==
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<StructureSection load='4gk9' size='340' side='right' caption='[[4gk9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='4gk9' size='340' side='right'caption='[[4gk9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gk9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_oklahomensis Burkholderia oklahomensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GK9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_oklahomensis Burkholderia oklahomensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GK9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gk9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gk9 RCSB], [http://www.ebi.ac.uk/pdbsum/4gk9 PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gk9 OCA], [https://pdbe.org/4gk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gk9 RCSB], [https://www.ebi.ac.uk/pdbsum/4gk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gk9 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/R4GRU1_9BURK R4GRU1_9BURK]
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Burkholderia oklahomensis EO147 agglutinin (BOA) is a 29 kDa member of the Oscillatoria agardhii agglutinin (OAA) family of lectins. Members of the OAA family recognize high-mannose glycans, and, by binding to the HIV envelope glycoprotein 120 (gp120), block the virus from binding to and entering the host cell, thereby inhibiting infection. OAA-family lectins comprise either one or two homologous domains, with a single domain possessing two glycan binding sites. We solved the structure of BOA in the ligand-free form as well as in complex with four molecules of 3alpha,6alpha-mannopentaose, the core unit of the N-linked high-mannose structures found on gp120 in vivo. This is the first structure of a double-domain OAA-family lectin in which all four binding sites are occupied by ligand. The structural details of the BOA-glycan interactions presented here, together with determination of affinity constants and HIV inactivation data, shed further light onto the structure-function relationship in this important class of anti-HIV proteins.
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Burkholderia oklahomensis agglutinin is a canonical two-domain OAA-family lectin: structures, carbohydrate binding and anti-HIV activity.,Whitley MJ, Furey W, Kollipara S, Gronenborn AM FEBS J. 2013 May;280(9):2056-67. doi: 10.1111/febs.12229. Epub 2013 Apr 2. PMID:23480609<ref>PMID:23480609</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Agglutinin|Agglutinin]]
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== References ==
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Burkholderia oklahomensis]]
[[Category: Burkholderia oklahomensis]]
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[[Category: Furey, W]]
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[[Category: Large Structures]]
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[[Category: Gronenborn, A M]]
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[[Category: Furey W]]
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[[Category: Whitley, M J]]
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[[Category: Gronenborn AM]]
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[[Category: Anti-hiv]]
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[[Category: Whitley MJ]]
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[[Category: Beta barrel]]
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[[Category: Glycan]]
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[[Category: Gp120]]
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[[Category: Lectin]]
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[[Category: Man9]]
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[[Category: Sugar binding]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of Burkholderia oklahomensis agglutinin (BOA) bound to 3a,6a-mannopentaose

PDB ID 4gk9

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