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| | ==Structure of apo Bradavidin2 (Form B)== | | ==Structure of apo Bradavidin2 (Form B)== |
| - | <StructureSection load='4ggt' size='340' side='right' caption='[[4ggt]], [[Resolution|resolution]] 1.69Å' scene=''> | + | <StructureSection load='4ggt' size='340' side='right'caption='[[4ggt]], [[Resolution|resolution]] 1.69Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ggt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ggt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GGT FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y32|2y32]], [[4ggr|4ggr]], [[4ggz|4ggz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.693Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bll1558 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 Bradyrhizobium japonicum USDA 110])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ggt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggt OCA], [https://pdbe.org/4ggt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ggt RCSB], [https://www.ebi.ac.uk/pdbsum/4ggt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ggt ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ggt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ggt RCSB], [http://www.ebi.ac.uk/pdbsum/4ggt PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q89U61_BRADU Q89U61_BRADU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4ggt" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Avidin|Avidin]] | + | *[[Avidin 3D structures|Avidin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bradyrhizobium japonicum usda 110]] | + | [[Category: Bradyrhizobium diazoefficiens USDA 110]] |
| - | [[Category: Livnah, O]] | + | [[Category: Large Structures]] |
| - | [[Category: Meir, A]] | + | [[Category: Livnah O]] |
| - | [[Category: Avidin]] | + | [[Category: Meir A]] |
| - | [[Category: Beta barrel]]
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| - | [[Category: Biotin binding protein]]
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| - | [[Category: Bradavidin]]
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| - | [[Category: High affinity system]]
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| - | [[Category: Lipocalin fold]]
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| - | [[Category: Oligomeric state]]
| + | |
| - | [[Category: Streptavidin]]
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| Structural highlights
Function
Q89U61_BRADU
Publication Abstract from PubMed
Bradavidin II is a biotin-binding protein from Bradyrhizobium japonicum that resembles chicken avidin and bacterial streptavidin. A biophysical characterization was carried out using dynamic light scattering, native mass spectrometry, differential scanning calorimetry, and isothermal titration calorimetry combined with structural characterization using X-ray crystallography. These observations revealed that bradavidin II differs from canonical homotetrameric avidin protein family members in its quaternary structure. In contrast with the other avidins, bradavidin II appears to have a dynamic (transient) oligomeric state in solution. It is monomeric at low protein concentrations but forms higher oligomeric assemblies at higher concentrations. The crystal structure of bradavidin II revealed an important role for Phe42 in shielding the bound ligand from surrounding water molecules, thus functionally replacing the L7,8 loop essential for tight ligand binding in avidin and streptavidin. This bradavidin II characterization opens new avenues for oligomerization-independent biotin-binding protein development.
The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.,Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP. The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins. Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323 doi:10.1002/pro.2281
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