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1rzu

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[[Image:1rzu.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the glycogen synthase from A. tumefaciens in complex with ADP==
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|PDB= 1rzu |SIZE=350|CAPTION= <scene name='initialview01'>1rzu</scene>, resolution 2.30&Aring;
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<StructureSection load='1rzu' size='340' side='right'caption='[[1rzu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[1rzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZU FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Starch_synthase Starch synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.21 2.4.1.21]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= GLGA1, GLGA, ATU4075, AGR_L_1562 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=358 Agrobacterium tumefaciens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzu OCA], [https://pdbe.org/1rzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rzu RCSB], [https://www.ebi.ac.uk/pdbsum/1rzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rzu ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the glycogen synthase from A. tumefaciens in complex with ADP'''
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== Function ==
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[https://www.uniprot.org/uniprot/GLGA1_RHIRD GLGA1_RHIRD] Synthesizes alpha-1,4-glucan chains using ADP-glucose.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Glycogen and starch are the major readily accessible energy storage compounds in nearly all living organisms. Glycogen is a very large branched glucose homopolymer containing about 90% alpha-1,4-glucosidic linkages and 10% alpha-1,6 linkages. Its synthesis and degradation constitute central pathways in the metabolism of living cells regulating a global carbon/energy buffer compartment. Glycogen biosynthesis involves the action of several enzymes among which glycogen synthase catalyzes the synthesis of the alpha-1,4-glucose backbone. We now report the first crystal structure of glycogen synthase in the presence and absence of adenosine diphosphate. The overall fold and the active site architecture of the protein are remarkably similar to those of glycogen phosphorylase, indicating a common catalytic mechanism and comparable substrate-binding properties. In contrast to glycogen phosphorylase, glycogen synthase has a much wider catalytic cleft, which is predicted to undergo an important interdomain 'closure' movement during the catalytic cycle. The structures also provide useful hints to shed light on the allosteric regulation mechanisms of yeast/mammalian glycogen synthases.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rzu_consurf.spt"</scriptWhenChecked>
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1RZU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZU OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation., Buschiazzo A, Ugalde JE, Guerin ME, Shepard W, Ugalde RA, Alzari PM, EMBO J. 2004 Aug 18;23(16):3196-205. Epub 2004 Jul 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272305 15272305]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rzu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Starch synthase]]
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[[Category: Alzari PM]]
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[[Category: Alzari, P M.]]
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[[Category: Buschiazzo A]]
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[[Category: Buschiazzo, A.]]
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[[Category: Guerin ME]]
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[[Category: Guerin, M E.]]
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[[Category: Shepard W]]
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[[Category: Shepard, W.]]
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[[Category: Ugalde JE]]
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[[Category: Ugalde, J E.]]
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[[Category: Ugalde RA]]
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[[Category: Ugalde, R A.]]
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[[Category: ADP]]
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[[Category: adp-binding]]
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[[Category: glycosyl-transferase]]
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[[Category: gt-b fold]]
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[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:58:34 2008''
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Current revision

Crystal structure of the glycogen synthase from A. tumefaciens in complex with ADP

PDB ID 1rzu

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