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| | ==Crystal structure of Saccharomyces cerevisiae Sfh3== | | ==Crystal structure of Saccharomyces cerevisiae Sfh3== |
| - | <StructureSection load='4j7p' size='340' side='right' caption='[[4j7p]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4j7p' size='340' side='right'caption='[[4j7p]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7P FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j7q|4j7q]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N1158, PDR16, SFH3, YNL231C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7p OCA], [https://pdbe.org/4j7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7p RCSB], [https://www.ebi.ac.uk/pdbsum/4j7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7p ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7p RCSB], [http://www.ebi.ac.uk/pdbsum/4j7p PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/PDR16_YEAST PDR16_YEAST] Has phosphatidylinositol transfer activity. Involved in the regulation of the phospholipid composition of plasma- and endomembranes. Altering plasma membrane composition may provide a possible mechanism for multidrug resistance. Involved in the regulation of sterol biosynthesis. Contributes to efficient phospholipase D1 activation in the regulation of phospholipid turnover.<ref>PMID:9890948</ref> <ref>PMID:10848624</ref> |
| - | Sec14 family homologs are the major yeast phosphatidylinositol/phosphatidylcholine transfer proteins regulating lipid metabolism and vesicle trafficking. The structure of Saccharomyces cerevisiae Sfh3 displays a conserved Sec14 scaffold and reveals determinants for the specific recognition of phosphatidylinositol ligand. Apo-Sfh3 forms a dimer through the hydrophobic interaction of gating helices. Binding of phosphatidylinositol leads to dissociation of the dimer into monomers in a reversible manner. This study suggests that the substrate induced dimer-monomer transformation is an essential part of lipid transfer cycles by Sfh3.
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| - | Structural determinants for phosphatidylinositol recognition by Sfh3 and substrate-induced dimer-monomer transition during lipid transfer cycles.,Yang H, Tong J, Leonard TA, Im YJ FEBS Lett. 2013 Jun 5;587(11):1610-6. doi: 10.1016/j.febslet.2013.04.009. Epub, 2013 Apr 18. PMID:23603387<ref>PMID:23603387</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Saccharomyces cerevisiae s288c]] | + | [[Category: Large Structures]] |
| - | [[Category: Im, Y J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Yang, H]] | + | [[Category: Im YJ]] |
| - | [[Category: Lipid transport]] | + | [[Category: Yang H]] |
| - | [[Category: Phosphatidylinositol]]
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| - | [[Category: Sec14 scaffold]]
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| Structural highlights
Function
PDR16_YEAST Has phosphatidylinositol transfer activity. Involved in the regulation of the phospholipid composition of plasma- and endomembranes. Altering plasma membrane composition may provide a possible mechanism for multidrug resistance. Involved in the regulation of sterol biosynthesis. Contributes to efficient phospholipase D1 activation in the regulation of phospholipid turnover.[1] [2]
References
- ↑ van den Hazel HB, Pichler H, do Valle Matta MA, Leitner E, Goffeau A, Daum G. PDR16 and PDR17, two homologous genes of Saccharomyces cerevisiae, affect lipid biosynthesis and resistance to multiple drugs. J Biol Chem. 1999 Jan 22;274(4):1934-41. PMID:9890948
- ↑ Li X, Routt SM, Xie Z, Cui X, Fang M, Kearns MA, Bard M, Kirsch DR, Bankaitis VA. Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth. Mol Biol Cell. 2000 Jun;11(6):1989-2005. PMID:10848624
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