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4ix5

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==Crystal structure of a Stt7 homolog from Micromonas algae in complex with AMP-PNP==
==Crystal structure of a Stt7 homolog from Micromonas algae in complex with AMP-PNP==
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<StructureSection load='4ix5' size='340' side='right' caption='[[4ix5]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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<StructureSection load='4ix5' size='340' side='right'caption='[[4ix5]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ix5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonas_sp._rcc299 Micromonas sp. rcc299]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IX5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ix5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonas_commoda Micromonas commoda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IX5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ix3|4ix3]], [[4ix4|4ix4]], [[4ix6|4ix6]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STT7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=296587 Micromonas sp. RCC299])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ix5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ix5 OCA], [https://pdbe.org/4ix5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ix5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ix5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ix5 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ix5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ix5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ix5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ix5 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C1EBN1_MICCC C1EBN1_MICCC]
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Under natural environments, plants and algae have evolved various photosynthetic acclimation mechanisms in response to the constantly changing light conditions. The state transition and long-term response processes in photosynthetic acclimation involve remodeling and composition alteration of thylakoid membrane. A chloroplast protein kinase named Stt7/STN7 has been found to have pivotal roles in both state transition and long-term response. Here we report the crystal structures of the kinase domain of a putative Stt7/STN7 homolog from Micromonas sp. RCC299 (MsStt7d) in the apo form and in complex with various nucleotide substrates. MsStt7d adopts a canonical protein kinase fold and contains all the essential residues at the active site. A novel hairpin motif, found to be a conserved feature of the Stt7/STN7 family and indispensable for the kinase stability, interacts with the activation loop and fixes it in an active conformation. We have also demonstrated that MsStt7d is a dualspecifi city kinase that phosphorylates both Thr and Tyr residues. Moreover, preliminary in vitro data suggest that it might be capable of phosphorylating a consensus N-terminal pentapeptide of light-harvesting proteins Micromonas Lhcp4 and Arabidopsis Lhcb1 directly. The potential peptide/protein substrate binding site is predicted based on the location of a pseudo-substrate contributed by the adjacent molecule within the crystallographic dimer. The structural and biochemical data presented here provide a framework for an improved understanding on the role of Stt7/STN7 in photosynthetic acclimation.
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Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae.,Guo J, Wei X, Li M, Pan X, Chang W, Liu Z Protein Cell. 2013 Aug;4(8):607-19. doi: 10.1007/s13238-013-3034-9. Epub 2013 Jun, 23. PMID:23794031<ref>PMID:23794031</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Micromonas sp. rcc299]]
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[[Category: Large Structures]]
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[[Category: Chang, W]]
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[[Category: Micromonas commoda]]
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[[Category: Guo, J]]
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[[Category: Chang W]]
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[[Category: Li, M]]
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[[Category: Guo J]]
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[[Category: Liu, Z]]
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[[Category: Li M]]
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[[Category: Pan, X]]
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[[Category: Liu Z]]
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[[Category: Wei, X]]
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[[Category: Pan X]]
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[[Category: Atp binding]]
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[[Category: Wei X]]
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[[Category: Canonical protein kinase fold]]
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[[Category: Protein kinase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of a Stt7 homolog from Micromonas algae in complex with AMP-PNP

PDB ID 4ix5

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