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| | ==Crystal Structure of a LSR-DNA Complex== | | ==Crystal Structure of a LSR-DNA Complex== |
| - | <StructureSection load='4kis' size='340' side='right' caption='[[4kis]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='4kis' size='340' side='right'caption='[[4kis]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4kis]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_innocua_clip11262 Listeria innocua clip11262]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4kis]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_innocua_Clip11262 Listeria innocua Clip11262]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KIS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">int ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272626 Listeria innocua Clip11262])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kis OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kis RCSB], [http://www.ebi.ac.uk/pdbsum/4kis PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kis OCA], [https://pdbe.org/4kis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kis RCSB], [https://www.ebi.ac.uk/pdbsum/4kis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kis ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q928V6_LISIN Q928V6_LISIN] |
| - | Serine integrases catalyze the integration of bacteriophage DNA into a host genome by site-specific recombination between 'attachment sites' in the phage (attP) and the host (attB). The reaction is highly directional; the reverse excision reaction between the product attL and attR sites does not occur in the absence of a phage-encoded factor, nor does recombination occur between other pairings of attachment sites. A mechanistic understanding of how these enzymes achieve site-selectivity and directionality has been limited by a lack of structural models. Here, we report the structure of the C-terminal domains of a serine integrase bound to an attP DNA half-site. The structure leads directly to models for understanding how the integrase-bound attP and attB sites differ, why these enzymes preferentially form attP x attB synaptic complexes to initiate recombination, and how attL x attR recombination is prevented. In these models, different domain organizations on attP vs. attB half-sites allow attachment-site specific interactions to form between integrase subunits via an unusual protruding coiled-coil motif. These interactions are used to preferentially synapse integrase-bound attP and attB and inhibit synapsis of integrase-bound attL and attR. The results provide a structural framework for understanding, testing and engineering serine integrase function.
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| - | Attachment site recognition and regulation of directionality by the serine integrases.,Rutherford K, Yuan P, Perry K, Sharp R, Van Duyne GD Nucleic Acids Res. 2013 Sep 1;41(17):8341-56. doi: 10.1093/nar/gkt580. Epub 2013 , Jul 2. PMID:23821671<ref>PMID:23821671</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Listeria innocua clip11262]] | + | [[Category: Large Structures]] |
| - | [[Category: Duyne, G D.Van]] | + | [[Category: Listeria innocua Clip11262]] |
| - | [[Category: Perry, K]] | + | [[Category: Perry K]] |
| - | [[Category: Rutherford, K]] | + | [[Category: Rutherford K]] |
| - | [[Category: Yuan, P]] | + | [[Category: Van Duyne GD]] |
| - | [[Category: Coiled-coil motif]] | + | [[Category: Yuan P]] |
| - | [[Category: Recombinase domain]]
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| - | [[Category: Recombination-dna complex]]
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| - | [[Category: Zinc-ribbon domain]]
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