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| | ==Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.== | | ==Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.== |
| - | <StructureSection load='4lu0' size='340' side='right' caption='[[4lu0]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='4lu0' size='340' side='right'caption='[[4lu0]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lu0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LU0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lu0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LU0 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-8-phosphooctulonate_synthase 3-deoxy-8-phosphooctulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.55 2.5.1.55] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lu0 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lu0 OCA], [https://pdbe.org/4lu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lu0 RCSB], [https://www.ebi.ac.uk/pdbsum/4lu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lu0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/KDSA_PSEAE KDSA_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4lu0" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 3-deoxy-8-phosphooctulonate synthase]] | + | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Asojo, O A]] | + | [[Category: Asojo OA]] |
| - | [[Category: Aldolase]]
| + | |
| - | [[Category: Arabinose 5 phosphate]]
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| - | [[Category: Kdo8p]]
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| - | [[Category: Phophoenol pyruvate]]
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| - | [[Category: Tim alpha-beta barrel]]
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| - | [[Category: Transferase]]
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| Structural highlights
Function
KDSA_PSEAE
Publication Abstract from PubMed
Pseudomonas aeruginosa is a major cause of opportunistic infection and is resistant to most antibiotics. As part of efforts to generate much-needed new antibiotics, structural studies of enzymes that are critical for the virulence of P. aeruginosa but are absent in mammals have been initiated. 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (KDO8Ps), also known as 2-dehydro-3-deoxyphosphooctonate aldolase, is vital for the survival and virulence of P. aeruginosa. This enzyme catalyzes a key step in the synthesis of the lipopolysaccharide (LPS) of most Gram-negative bacteria: the condensation reaction between phosphoenolpyruvate (PEP) and arabinose 5-phosphate to produce 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate (KDO8P). This step is vital for the proper synthesis and assembly of LPS and the survival of P. aeruginosa. Here, the recombinant expression, purification and crystal structure of KDO8Ps from P. aeruginosa are presented. Orthorhombic crystals were obtained by vapor diffusion in sitting drops in the presence of 1 mM phosphoenlpyruvate. The structure reveals the prototypical alpha/beta TIM-barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit.
Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.,Nelson SK, Kelleher A, Robinson G, Reiling S, Asojo OA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1084-8. doi:, 10.1107/S1744309113023993. Epub 2013 Sep 28. PMID:24100553[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nelson SK, Kelleher A, Robinson G, Reiling S, Asojo OA. Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1084-8. doi:, 10.1107/S1744309113023993. Epub 2013 Sep 28. PMID:24100553 doi:http://dx.doi.org/10.1107/S1744309113023993
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