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4jy8

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==X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters==
==X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters==
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<StructureSection load='4jy8' size='340' side='right' caption='[[4jy8]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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<StructureSection load='4jy8' size='340' side='right'caption='[[4jy8]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4jy8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JY8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4jy8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JY8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.901&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iix|3iix]], [[3ciw|3ciw]], [[4jxc|4jxc]], [[4jy9|4jy9]], [[4jyd|4jyd]], [[4jye|4jye]], [[4jyf|4jyf]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ThemaDRAFT_0043, TM1269, TM_1269 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 Thermotoga maritima MSB8])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jy8 OCA], [https://pdbe.org/4jy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jy8 RCSB], [https://www.ebi.ac.uk/pdbsum/4jy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jy8 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jy8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jy8 RCSB], [http://www.ebi.ac.uk/pdbsum/4jy8 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/HYDE_THEMA HYDE_THEMA] Required for the maturation of the [FeFe]-hydrogenase HydA (By similarity). Catalyzes the reductive cleavage of S-adenosyl-L-methionine (in vitro), suggesting it may contribute to the biosynthesis of an essential sulfur-containing ligand that binds to the hydrogenase active site [2Fe-2S] cluster (PubMed:16137685).[UniProtKB:Q97IK9]<ref>PMID:16137685</ref>
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Fe4S4 clusters are very common versatile prosthetic groups in proteins. Their redox property of being sensitive to O2-induced oxidative damage is, for instance, used by the cell to sense oxygen levels and switch between aerobic and anaerobic metabolisms, as exemplified by the fumarate, nitrate reduction regulator (FNR). Using the hydrogenase maturase HydE from Thermotoga maritima as a template, we obtained several unusual forms of FeS clusters, some of which are associated with important structural changes. These structures represent intermediate states relevant to both FeS cluster assembly and degradation. We observe one Fe2S2 cluster bound by two cysteine persulfide residues. This observation lends structural support to a very recent Raman study, which reported that Fe4S4-to-Fe2S2 cluster conversion upon oxygen exposure in FNR resulted in concomitant production of cysteine persulfide as cluster ligands. Similar persulfide ligands have been observed in vitro for several other Fe4S4 cluster-containing proteins. We have also monitored FeS cluster conversion directly in our protein crystals. Our structures indicate that the Fe4S4-to-Fe2S2 change requires large structural modifications, which are most likely responsible for the dimer-monomer transition in FNR.
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X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.,Nicolet Y, Rohac R, Martin L, Fontecilla-Camps JC Proc Natl Acad Sci U S A. 2013 Apr 17. PMID:23596207<ref>PMID:23596207</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Thermotoga maritima msb8]]
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[[Category: Large Structures]]
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[[Category: Fontecilla-Camps, J C]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Martin, L]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Nicolet, Y]]
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[[Category: Martin L]]
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[[Category: Rohac, R]]
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[[Category: Nicolet Y]]
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[[Category: Fe4s4 cluster]]
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[[Category: Rohac R]]
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[[Category: Fefe-hydrogenase active site assembly]]
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[[Category: Oxidoreductase]]
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[[Category: Radical sam protein]]
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[[Category: S-adenosyl-l-methionine]]
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Current revision

X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters

PDB ID 4jy8

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