This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4lz6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:55, 20 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Structure of MATE multidrug transporter DinF-BH==
==Structure of MATE multidrug transporter DinF-BH==
-
<StructureSection load='4lz6' size='340' side='right' caption='[[4lz6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+
<StructureSection load='4lz6' size='340' side='right'caption='[[4lz6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4lz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LZ6 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4lz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LZ6 FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lz9|4lz9]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BH2163 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272558 BACHD])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lz6 OCA], [https://pdbe.org/4lz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lz6 RCSB], [https://www.ebi.ac.uk/pdbsum/4lz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lz6 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lz6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lz6 RCSB], [http://www.ebi.ac.uk/pdbsum/4lz6 PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q9KAX3_HALH5 Q9KAX3_HALH5]
-
Multidrug and toxic compound extrusion (MATE) transporters contribute to multidrug resistance by coupling the efflux of drugs to the influx of Na(+) or H(+). Known structures of Na(+)-coupled, extracellular-facing MATE transporters from the NorM subfamily revealed 12 membrane-spanning segments related by a quasi-two-fold rotational symmetry and a multidrug-binding cavity situated near the membrane surface. Here we report the crystal structure of an H(+)-coupled MATE transporter from Bacillus halodurans and the DinF subfamily at 3.2-A resolution, unveiling a surprisingly asymmetric arrangement of 12 transmembrane helices. We also identified a membrane-embedded substrate-binding chamber by combining crystallographic and biochemical analyses. Our studies further suggested a direct competition between H(+) and substrate during DinF-mediated transport and implied how a MATE transporter alternates between its extracellular- and intracellular-facing conformations to propel multidrug extrusion. Collectively, our results demonstrated heretofore-unrecognized mechanistic diversity among MATE transporters.
+
-
 
+
-
Structural insights into H(+)-coupled multidrug extrusion by a MATE transporter.,Lu M, Radchenko M, Symersky J, Nie R, Guo Y Nat Struct Mol Biol. 2013 Nov;20(11):1310-7. doi: 10.1038/nsmb.2687. Epub 2013, Oct 20. PMID:24141706<ref>PMID:24141706</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bachd]]
+
[[Category: Alkalihalobacillus halodurans C-125]]
-
[[Category: Guo, Y]]
+
[[Category: Large Structures]]
-
[[Category: Lu, M]]
+
[[Category: Guo Y]]
-
[[Category: Nie, R]]
+
[[Category: Lu M]]
-
[[Category: Radchenko, M]]
+
[[Category: Nie R]]
-
[[Category: Symersky, J]]
+
[[Category: Radchenko M]]
-
[[Category: Multidrug transporter]]
+
[[Category: Symersky J]]
-
[[Category: Transport protein]]
+

Current revision

Structure of MATE multidrug transporter DinF-BH

PDB ID 4lz6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lz6"
Personal tools