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| | ==Crystal structure of Mycobacterium tuberculosis CarD== | | ==Crystal structure of Mycobacterium tuberculosis CarD== |
| - | <StructureSection load='4mfr' size='340' side='right' caption='[[4mfr]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4mfr' size='340' side='right'caption='[[4mfr]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MFR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MFR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ilu|4ilu]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">carD, Rv3583c, MT3689 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfr OCA], [https://pdbe.org/4mfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mfr RCSB], [https://www.ebi.ac.uk/pdbsum/4mfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mfr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mfr RCSB], [http://www.ebi.ac.uk/pdbsum/4mfr PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4mfr" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Mycobacterium tuberculosis]] | | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Kaur, G]] | + | [[Category: Kaur G]] |
| - | [[Category: Thakur, K G]] | + | [[Category: Thakur KG]] |
| - | [[Category: Five helical bundle]]
| + | |
| - | [[Category: Rna polymerase]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcriptional regulator]]
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| - | [[Category: Tudor-like domain]]
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| Structural highlights
Function
CARD_MYCTU Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.[1] [2]
Publication Abstract from PubMed
Mycobacterium tuberculosis (Mtb) CarD is an essential transcriptional regulator that binds RNA polymerase and plays an important role in reprogramming transcription machinery under diverse stress conditions. Here, we report the crystal structure of CarD at 2.3 A resolution, that represents the first structural description of CarD/CdnL-Like family of proteins. CarD adopts an overall bi-lobed structural architecture where N-terminal domain resembles 'tudor-like' domain and C-terminal domain adopts a novel five helical fold that lacks the predicted leucine zipper structural motif. The structure reveals dimeric state of CarD resulting from beta-strand swapping between the N-terminal domains of each individual subunits. The structure provides crucial insights into the possible mode(s) of CarD/RNAP interactions. Proteins 2013; (c) 2013 Wiley Periodicals, Inc.
Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture.,Kaur G, Dutta D, Thakur KG Proteins. 2013 Sep 30. doi: 10.1002/prot.24419. PMID:24115125[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stallings CL, Stephanou NC, Chu L, Hochschild A, Nickels BE, Glickman MS. CarD is an essential regulator of rRNA transcription required for Mycobacterium tuberculosis persistence. Cell. 2009 Jul 10;138(1):146-59. doi: 10.1016/j.cell.2009.04.041. PMID:19596241 doi:10.1016/j.cell.2009.04.041
- ↑ Weiss LA, Harrison PG, Nickels BE, Glickman MS, Campbell EA, Darst SA, Stallings CL. Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis. J Bacteriol. 2012 Oct;194(20):5621-31. Epub 2012 Aug 17. PMID:22904282 doi:10.1128/JB.00879-12
- ↑ Kaur G, Dutta D, Thakur KG. Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture. Proteins. 2013 Sep 30. doi: 10.1002/prot.24419. PMID:24115125 doi:http://dx.doi.org/10.1002/prot.24419
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