4nuf

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==Crystal Structure of SHP/EID1==
==Crystal Structure of SHP/EID1==
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<StructureSection load='4nuf' size='340' side='right' caption='[[4nuf]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='4nuf' size='340' side='right'caption='[[4nuf]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4nuf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NUF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4nuf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O104:H4_str._2009EL-2071 Escherichia coli O104:H4 str. 2009EL-2071] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NUF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">malE, malE O3O_01660, Nr0b2, Shp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nuf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nuf RCSB], [http://www.ebi.ac.uk/pdbsum/4nuf PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nuf OCA], [https://pdbe.org/4nuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nuf RCSB], [https://www.ebi.ac.uk/pdbsum/4nuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nuf ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/NR0B2_MOUSE NR0B2_MOUSE] Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.<ref>PMID:8650544</ref>
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Small heterodimer partner (SHP) is an orphan nuclear receptor that functions as a transcriptional repressor to regulate bile acid and cholesterol homeostasis. Although the precise mechanism whereby SHP represses transcription is not known, E1A-like inhibitor of differentiation (EID1) was isolated as a SHP-interacting protein and implicated in SHP repression. Here we present the crystal structure of SHP in complex with EID1, which reveals an unexpected EID1-binding site on SHP. Unlike the classical cofactor-binding site near the C-terminal helix H12, the EID1-binding site is located at the N terminus of the receptor, where EID1 mimics helix H1 of the nuclear receptor ligand-binding domain. The residues composing the SHP-EID1 interface are highly conserved. Their mutation diminishes SHP-EID1 interactions and affects SHP repressor activity. Together, these results provide important structural insights into SHP cofactor recruitment and repressor function and reveal a conserved protein interface that is likely to have broad implications for transcriptional repression by orphan nuclear receptors.
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Structural insights into gene repression by the orphan nuclear receptor SHP.,Zhi X, Zhou XE, He Y, Zechner C, Suino-Powell KM, Kliewer SA, Melcher K, Mangelsdorf DJ, Xu HE Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):839-44. doi:, 10.1073/pnas.1322827111. Epub 2013 Dec 30. PMID:24379397<ref>PMID:24379397</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lk3 transgenic mice]]
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[[Category: Escherichia coli O104:H4 str. 2009EL-2071]]
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[[Category: He, Y]]
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[[Category: Large Structures]]
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[[Category: Kliewer, S A]]
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[[Category: Mus musculus]]
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[[Category: Mangelsdorf, D J]]
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[[Category: He Y]]
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[[Category: Melcher, K]]
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[[Category: Kliewer SA]]
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[[Category: Suino-Powell, K M]]
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[[Category: Mangelsdorf DJ]]
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[[Category: Xu, H E]]
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[[Category: Melcher K]]
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[[Category: Zechner, C]]
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[[Category: Suino-Powell KM]]
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[[Category: Zhi, X]]
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[[Category: Xu HE]]
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[[Category: Zhou, X E]]
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[[Category: Zechner C]]
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[[Category: Peptide mimicking protein helix]]
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[[Category: Zhi X]]
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[[Category: Protein-peptide complex]]
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[[Category: Zhou XE]]
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[[Category: Sandwich fold]]
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[[Category: Transcription]]
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[[Category: Transport protein]]
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Current revision

Crystal Structure of SHP/EID1

PDB ID 4nuf

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