3ite
From Proteopedia
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==The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase== | ==The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase== | ||
| - | <StructureSection load='3ite' size='340' side='right' caption='[[3ite]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3ite' size='340' side='right'caption='[[3ite]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3ite]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3ite]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Epichloe_festucae_var._lolii Epichloe festucae var. lolii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ITE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ITE FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | < | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ite FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ite OCA], [https://pdbe.org/3ite PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ite RCSB], [https://www.ebi.ac.uk/pdbsum/3ite PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ite ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SIDN_EPIFI SIDN_EPIFI] Nonribosomal peptide synthase required for the biosynthetis of epichloenin A, an extracellular siderophore that plays a crucial role in endophyte-grass symbioses (PubMed:19923209, PubMed:23658520). SidN assembles epichloenin A by activating and incorporating three trans-anhydromevalonylhydroxyornithine (trans-AMHO), 1 glutamine and 4 glycine moieties (PubMed:23658520). Trans-AMHO is produced from L-ornithine via 2 steps involving a L-ornithine N(5)-monooxygenase and an AHMO-N(5)-transacylase that have still to be identified (PubMed:19923209). The third adenylation domain (A3) of sidN incorporates the hydroxamate groups of the siderophore which forms an octahedral iron complex (PubMed:19923209). The other component amino acids are assembled by sidN adenylation domains A1 and A2 (PubMed:19923209, PubMed:23658520).<ref>PMID:19923209</ref> <ref>PMID:23658520</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3ite_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3ite_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ite ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
| + | <div class="pdbe-citations 3ite" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Epichloe festucae var. lolii]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Arcus VL]] |
| - | [[Category: Johnson | + | [[Category: Johnson LJ]] |
| - | [[Category: | + | [[Category: Johnson RD]] |
| - | [[Category: | + | [[Category: Lee TV]] |
| - | [[Category: | + | [[Category: Lott JS]] |
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Current revision
The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase
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