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1suu

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[[Image:1suu.gif|left|200px]]
 
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{{Structure
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==Structure of DNA gyrase A C-terminal domain==
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|PDB= 1suu |SIZE=350|CAPTION= <scene name='initialview01'>1suu</scene>, resolution 1.75&Aring;
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<StructureSection load='1suu' size='340' side='right'caption='[[1suu]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1suu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SUU FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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|GENE= GYRA, BB0435 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=139 Borrelia burgdorferi])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1suu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1suu OCA], [https://pdbe.org/1suu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1suu RCSB], [https://www.ebi.ac.uk/pdbsum/1suu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1suu ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GYRA_BORBU GYRA_BORBU] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/su/1suu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1suu ConSurf].
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<div style="clear:both"></div>
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'''Structure of DNA gyrase A C-terminal domain'''
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==See Also==
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*[[Gyrase 3D Structures|Gyrase 3D Structures]]
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__TOC__
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==Overview==
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</StructureSection>
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DNA gyrase is unique among enzymes for its ability to actively introduce negative supercoils into DNA. This function is mediated in part by the C-terminal domain of its A subunit (GyrA CTD). Here, we report the crystal structure of this approximately 35-kDa domain determined to 1.75-A resolution. The GyrA CTD unexpectedly adopts an unusual fold, which we term a beta-pinwheel, that is globally reminiscent of a beta-propeller but is built of blades with a previously unobserved topology. A large, conserved basic patch on the outer edge of this domain suggests a likely site for binding and bending DNA; fluorescence resonance energy transfer-based assays show that the GyrA CTD is capable of bending DNA by &gt; or =180 degrees over a 40-bp region. Surprisingly, we find that the CTD of the topoisomerase IV A subunit, which shares limited sequence homology with the GyrA CTD, also bends DNA. Together, these data provide a physical explanation for the ability of DNA gyrase to constrain a positive superhelical DNA wrap, and also suggest that the particular substrate preferences of topoisomerase IV might be dictated in part by the function of this domain.
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[[Category: Borreliella burgdorferi]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Berger JM]]
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1SUU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SUU OCA].
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[[Category: Corbett KD]]
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[[Category: Shultzaberger RK]]
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==Reference==
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The C-terminal domain of DNA gyrase A adopts a DNA-bending beta-pinwheel fold., Corbett KD, Shultzaberger RK, Berger JM, Proc Natl Acad Sci U S A. 2004 May 11;101(19):7293-8. Epub 2004 May 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15123801 15123801]
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[[Category: Borrelia burgdorferi]]
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[[Category: DNA topoisomerase (ATP-hydrolyzing)]]
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[[Category: Single protein]]
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[[Category: Berger, J M.]]
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[[Category: Corbett, K D.]]
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[[Category: Shultzaberger, R K.]]
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[[Category: beta-pinwheel]]
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[[Category: beta-propeller]]
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[[Category: dna gyrase]]
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[[Category: topoisomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:09:54 2008''
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Current revision

Structure of DNA gyrase A C-terminal domain

PDB ID 1suu

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