This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4kl0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:54, 20 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the effector protein XOO4466==
==Crystal structure of the effector protein XOO4466==
-
<StructureSection load='4kl0' size='340' side='right' caption='[[4kl0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+
<StructureSection load='4kl0' size='340' side='right'caption='[[4kl0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4kl0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanor Xanor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KL0 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4kl0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_oryzae_pv._oryzae_KACC_10331 Xanthomonas oryzae pv. oryzae KACC 10331]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KL0 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.598&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XOO4466 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=291331 XANOR])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kl0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kl0 RCSB], [http://www.ebi.ac.uk/pdbsum/4kl0 PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kl0 OCA], [https://pdbe.org/4kl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kl0 RCSB], [https://www.ebi.ac.uk/pdbsum/4kl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kl0 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q5GUA3_XANOR Q5GUA3_XANOR]
-
Many Gram-negative bacteria deliver their virulence factors into host cells through a secretion system. Those factors, called effector proteins, are involved in the pathogenicity in host cells by interfering with various cellular events. The phytopathogen Xanthomonas oryzae pv. oryzae uses a type III secretion system to inject its effectors, but the functional roles of these proteins remain largely uncharacterized. Here, we determined a crystal structure of XOO4466, an effector from X. oryzae pv. oryzae, and performed a functional analysis. We determined that XOO4466 is similar in sequence to Xanthomonas outer protein Q, a putative nucleoside hydrolase (NH). The overall structure of XOO4466 is homologous to that of NHs, including a metal-binding site, but differs in its oligomeric state and active site topology. Further analysis indicated that antiparallel beta-strands commonly found in NHs adjacent to the active site loop are replaced in XOO4466 with a short loop, causing the active site loop to adopt a conformation distinct from that of NHs. Thus, the catalytic residues emanating from the respective active site loop of NHs are absent in the putative active site of XOO4466. Consistent with these structural features, a functional assay indicated that XOO4466 does not exhibit NH activity and possibly catalyzes yet unknown reactions.
+
-
 
+
-
Crystal structure of the effector protein XOO4466 from Xanthomonas oryzae.,Yu S, Hwang I, Rhee S J Struct Biol. 2013 Sep 3. pii: S1047-8477(13)00213-X. doi:, 10.1016/j.jsb.2013.08.007. PMID:24007778<ref>PMID:24007778</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Xanor]]
+
[[Category: Large Structures]]
-
[[Category: Rhee, S]]
+
[[Category: Xanthomonas oryzae pv. oryzae KACC 10331]]
-
[[Category: Yu, S]]
+
[[Category: Rhee S]]
-
[[Category: Calcium binding protein]]
+
[[Category: Yu S]]
-
[[Category: Rossmann fold]]
+
-
[[Category: Unknown function]]
+

Current revision

Crystal structure of the effector protein XOO4466

PDB ID 4kl0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kl0"
Personal tools