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3wnk

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==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==
==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==
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<StructureSection load='3wnk' size='340' side='right' caption='[[3wnk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3wnk' size='340' side='right'caption='[[3wnk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WNK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wnl|3wnl]], [[3wnm|3wnm]], [[3wnn|3wnn]], [[3wno|3wno]], [[3wnp|3wnp]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [https://pdbe.org/3wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [https://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CTA1_NIACI CTA1_NIACI] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
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Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His(6) tag diffracted to a resolution of 2.3 A and belonged to space group P3(1)21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 A and belonged to space group P2(1)2(1)2(1), containing two molecules in the asymmetric unit.
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Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050<ref>PMID:23908050</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 24]]
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[[Category: Large Structures]]
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[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
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[[Category: Niallia circulans]]
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[[Category: Fujimoto, Z]]
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[[Category: Fujimoto Z]]
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[[Category: Funane, K]]
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[[Category: Funane K]]
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[[Category: Kim, Y M]]
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[[Category: Kim YM]]
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[[Category: Kimura, A]]
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[[Category: Kimura A]]
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[[Category: Kishine, N]]
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[[Category: Kishine N]]
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[[Category: Kobayashi, M]]
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[[Category: Kobayashi M]]
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[[Category: Momma, M]]
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[[Category: Momma M]]
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[[Category: Suzuki, N]]
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[[Category: Suzuki N]]
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[[Category: Suzuki, R]]
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[[Category: Suzuki R]]
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[[Category: 6-glucan]]
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[[Category: Alpha-1]]
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[[Category: Beta-jelly roll]]
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[[Category: C2 type immunoglobulin fold]]
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[[Category: Glycoside hydrolase]]
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[[Category: Greek key]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase

PDB ID 3wnk

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