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1t8s

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[[Image:1t8s.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate==
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|PDB= 1t8s |SIZE=350|CAPTION= <scene name='initialview01'>1t8s</scene>, resolution 2.60&Aring;
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<StructureSection load='1t8s' size='340' side='right'caption='[[1t8s]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMP:FORMYCIN-5'-MONOPHOSPHATE'>FMP</scene>
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<table><tr><td colspan='2'>[[1t8s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8S FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/AMP_nucleosidase AMP nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.4 3.2.2.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE= AMN, B1982, Z3139, ECS2779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMP:FORMYCIN-5-MONOPHOSPHATE'>FMP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8s OCA], [https://pdbe.org/1t8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8s RCSB], [https://www.ebi.ac.uk/pdbsum/1t8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8s ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate'''
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== Function ==
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[https://www.uniprot.org/uniprot/AMN_ECOLI AMN_ECOLI] Involved in regulation of AMP concentrations.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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AMP nucleosidase (AMN) catalyzes the hydrolysis of AMP to form adenine and ribose 5-phosphate. The enzyme is found only in prokaryotes, where it plays a role in purine nucleoside salvage and intracellular AMP level regulation. Enzyme activity is stimulated by ATP and suppressed by phosphate. The structure of unliganded AMN was determined at 2.7 A resolution, and structures of the complexes with either formycin 5'-monophosphate or inorganic phosphate were determined at 2.6 A and 3.0 A resolution, respectively. AMN is a biological homohexamer, and each monomer is composed of two domains: a catalytic domain and a putative regulatory domain. The overall topology of the catalytic domain and some features of the substrate binding site resemble those of the nucleoside phosphorylases, demonstrating that AMN is a new member of the family. The structure of the regulatory domain consists of a long helix and a four-stranded sheet and has a novel topology.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8s_consurf.spt"</scriptWhenChecked>
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1T8S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8S OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of Escherichia coli AMP nucleosidase reveals similarity to nucleoside phosphorylases., Zhang Y, Cottet SE, Ealick SE, Structure. 2004 Aug;12(8):1383-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15296732 15296732]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8s ConSurf].
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[[Category: AMP nucleosidase]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cottet, S E.]]
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[[Category: Cottet SE]]
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[[Category: Ealick, S E.]]
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[[Category: Ealick SE]]
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[[Category: Zhang, Y.]]
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[[Category: Zhang Y]]
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[[Category: FMP]]
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[[Category: alpha-beta fold]]
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[[Category: alpha-beta-alpha sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:24 2008''
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Current revision

Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate

PDB ID 1t8s

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