2cc3

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[[Image:2cc3.gif|left|200px]]<br />
 
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<applet load="2cc3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cc3, resolution 2.20&Aring;" />
 
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'''STRUCTURE OF AGROBACTERIUM TUMEFACIENS VIRB8 PROTEIN'''<br />
 
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==Overview==
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==Structure of Agrobacterium tumefaciens VirB8 protein==
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Bacterial type IV secretion systems (T4SS) translocate DNA and/or proteins, to recipient cells, thus providing a mechanism for conjugative transfer of, genetic material and bacterial pathogenesis. Here we describe the first, structure of a core component from the archetypal Agrobacterium, tumefaciens T4SS: the 2.2-A resolution crystal structure of the VirB8, periplasmic domain (pVirB8(AT)). VirB8 forms a dimer in the crystal, and, we identify residues likely important for stabilization of the dimer, interface. Structural comparison of pVirB8(AT) with Brucella suis VirB8, confirms that the monomers have a similar fold. In addition, the, pVirB8(AT) dimer superimposes very closely on the B. suis VirB8 dimer, supporting the proposal that dimer formation in the crystal reflects, self-interactions that are biologically significant. The evolutionary, conservation level for each residue was obtained from a data set of 84, VirB8 homologs and projected onto the protein structure to indicate, conserved surface patches that likely contact other T4SS proteins.
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<StructureSection load='2cc3' size='340' side='right'caption='[[2cc3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cc3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CC3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cc3 OCA], [https://pdbe.org/2cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/2cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cc3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VIRB8_AGRFC VIRB8_AGRFC] VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/2cc3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cc3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial type IV secretion systems (T4SS) translocate DNA and/or proteins to recipient cells, thus providing a mechanism for conjugative transfer of genetic material and bacterial pathogenesis. Here we describe the first structure of a core component from the archetypal Agrobacterium tumefaciens T4SS: the 2.2-A resolution crystal structure of the VirB8 periplasmic domain (pVirB8(AT)). VirB8 forms a dimer in the crystal, and we identify residues likely important for stabilization of the dimer interface. Structural comparison of pVirB8(AT) with Brucella suis VirB8 confirms that the monomers have a similar fold. In addition, the pVirB8(AT) dimer superimposes very closely on the B. suis VirB8 dimer, supporting the proposal that dimer formation in the crystal reflects self-interactions that are biologically significant. The evolutionary conservation level for each residue was obtained from a data set of 84 VirB8 homologs and projected onto the protein structure to indicate conserved surface patches that likely contact other T4SS proteins.
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==About this Structure==
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Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites.,Bailey S, Ward D, Middleton R, Grossmann JG, Zambryski PC Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2582-7. Epub 2006 Feb 15. PMID:16481621<ref>PMID:16481621</ref>
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2CC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CC3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites., Bailey S, Ward D, Middleton R, Grossmann JG, Zambryski PC, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2582-7. Epub 2006 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16481621 16481621]
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</div>
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<div class="pdbe-citations 2cc3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bailey, S.]]
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[[Category: Bailey S]]
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[[Category: Grossmann, G.]]
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[[Category: Grossmann G]]
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[[Category: Middleton, R.]]
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[[Category: Middleton R]]
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[[Category: Ward, D.]]
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[[Category: Ward D]]
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[[Category: Zambryski, P.C.]]
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[[Category: Zambryski PC]]
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[[Category: MPD]]
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[[Category: agrobacterium]]
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[[Category: consurf]]
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[[Category: type iv secretion system]]
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[[Category: virb8]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:49:03 2007''
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Current revision

Structure of Agrobacterium tumefaciens VirB8 protein

PDB ID 2cc3

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