2jtb
From Proteopedia
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==Three dimensional solution structure of hainantoxin-III by 2D 1H-NMR== | ==Three dimensional solution structure of hainantoxin-III by 2D 1H-NMR== | ||
| - | <StructureSection load='2jtb' size='340' side='right' caption='[[2jtb | + | <StructureSection load='2jtb' size='340' side='right'caption='[[2jtb]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2jtb]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2jtb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haplopelma_hainanum Haplopelma hainanum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JTB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jtb OCA], [https://pdbe.org/2jtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jtb RCSB], [https://www.ebi.ac.uk/pdbsum/2jtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jtb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/H3A01_CYRHA H3A01_CYRHA] Selective antagonist of neuronal tetrodotoxin (TTX)-sensitive voltage-gated sodium channels (IC(50)=1270 nM on Nav1.1/SCN1A, 270 nM on Nav1.2/SCN2A, 491 nM on Nav1.3/SCN3A and 232 nM on Nav1.7/SCN9A). This toxin suppress Nav1.7 current amplitude without significantly altering the activation, inactivation, and repriming kinetics. Short extreme depolarizations partially activate the toxin-bound channel, indicating voltage-dependent inhibition of this toxin. This toxin increases the deactivation of the Nav1.7 current after extreme depolarizations. The toxin-Nav1.7 complex is gradually dissociated upon prolonged strong depolarizations in a voltage-dependent manner, and the unbound toxin rebinds to Nav1.7 after a long repolarization. Moreover, analysis of chimeric channels showed that the DIIS3-S4 linker is critical for toxin binding to Nav1.7. These data are consistent with this toxin interacting with Nav1.7 site 4 and trapping the domain II voltage sensor in the closed state.<ref>PMID:23703613</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Haplopelma hainanum]] | [[Category: Haplopelma hainanum]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Liang S]] |
| - | [[Category: | + | [[Category: Liu Z]] |
| - | [[Category: | + | [[Category: Zhu Q]] |
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Current revision
Three dimensional solution structure of hainantoxin-III by 2D 1H-NMR
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