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2c5i

From Proteopedia

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N-terminal domain of tlg1 complexed with N-terminus of vps51 in distorted conformation

Structural highlights

2c5i is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VPS51_YEAST Involved in retrograde transport from early and late endosomes to late Golgi. Links the Golgi-associated retrograde protein (GARP) complex to the t-SNARE TLG1, leading to the fusion between the endosomal vesicles with the late Golgi. May also be indirectly involved in apical bud growth.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

Membrane fusion in cells involves the interaction of SNARE proteins on apposing membranes. Formation of SNARE complexes is preceded by tethering events, and a number of protein complexes that are thought to mediate this have been identified. The VFT or GARP complex is required for endosome-Golgi traffic in yeast. It consists of four subunits, one of which, Vps51, has been shown to bind specifically to the SNARE Tlg1, which participates in the same fusion event. We have determined the structure of the N-terminal domain of Tlg1 bound to a peptide from the N terminus of Vps51. Binding depends mainly on residues 18-30 of Vps51. These form a short helix which lies in a conserved groove in the three-helix bundle formed by Tlg1. Surprisingly, although both Vps51 and Tlg1 are required for transport to the late Golgi from endosomes, removal of the Tlg1-binding sequences from Vps51 does not block such traffic in vivo. Thus, this particular interaction cannot be crucial to the process of vesicle docking or fusion.

Structural analysis of the interaction between the SNARE Tlg1 and Vps51.,Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR Traffic. 2006 Feb;7(2):182-90. PMID:16420526^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002 Apr;1(6):345-56. Epub 2001 Dec 21. PMID:11957109 doi:http://dx.doi.org/10.1007/s10142-001-0043-1
↑ Siniossoglou S, Pelham HR. Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem. 2002 Dec 13;277(50):48318-24. Epub 2002 Oct 10. PMID:12377769 doi:10.1074/jbc.M209428200
↑ Bonangelino CJ, Chavez EM, Bonifacino JS. Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell. 2002 Jul;13(7):2486-501. PMID:12134085 doi:http://dx.doi.org/10.1091/mbc.02-01-0005
↑ Reggiori F, Wang CW, Stromhaug PE, Shintani T, Klionsky DJ. Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem. 2003 Feb 14;278(7):5009-20. Epub 2002 Nov 20. PMID:12446664 doi:10.1074/jbc.M210436200
↑ Conibear E, Cleck JN, Stevens TH. Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell. 2003 Apr;14(4):1610-23. PMID:12686613 doi:10.1091/mbc.E02-10-0654
↑ Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR. Structural analysis of the interaction between the SNARE Tlg1 and Vps51. Traffic. 2006 Feb;7(2):182-90. PMID:16420526 doi:TRA374

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c5i"

@@ Line 1: / Line 1: @@
-==N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51 IN DISTORTED CONFORMATION==
-<StructureSection load='2c5i' size='340' side='right' caption='[[2c5i]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+==N-terminal domain of tlg1 complexed with N-terminus of vps51 in distorted conformation==
+<StructureSection load='2c5i' size='340' side='right'caption='[[2c5i]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2c5i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C5I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2c5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C5I FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2c5j|2c5j]], [[2c5k|2c5k]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5i OCA], [https://pdbe.org/2c5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c5i RCSB], [https://www.ebi.ac.uk/pdbsum/2c5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c5i ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c5i RCSB], [http://www.ebi.ac.uk/pdbsum/2c5i PDBsum]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/VPS51_YEAST VPS51_YEAST] Involved in retrograde transport from early and late endosomes to late Golgi. Links the Golgi-associated retrograde protein (GARP) complex to the t-SNARE TLG1, leading to the fusion between the endosomal vesicles with the late Golgi. May also be indirectly involved in apical bud growth.<ref>PMID:11957109</ref> <ref>PMID:12377769</ref> <ref>PMID:12134085</ref> <ref>PMID:12446664</ref> <ref>PMID:12686613</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2c5i" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Evans, P R]]
+[[Category: Evans PR]]
-[[Category: Fridmann-Sirkis, Y]]
+[[Category: Fridmann-Sirkis Y]]
-[[Category: Kent, H M]]
+[[Category: Kent HM]]
-[[Category: Lewis, M J]]
+[[Category: Lewis MJ]]
-[[Category: Pelham, H R.B]]
+[[Category: Pelham HRB]]
-[[Category: Phosphorylation]]
-[[Category: Protein transport]]
-[[Category: Protein transport-complex]]
-[[Category: Snare]]
-[[Category: Vft complex]]

2c5i

From Proteopedia

Current revision

N-terminal domain of tlg1 complexed with N-terminus of vps51 in distorted conformation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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