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1tkl

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[[Image:1tkl.jpg|left|200px]]
 
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{{Structure
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==Yeast Oxygen-Dependent Coproporphyrinogen Oxidase==
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|PDB= 1tkl |SIZE=350|CAPTION= <scene name='initialview01'>1tkl</scene>, resolution 2.00&Aring;
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<StructureSection load='1tkl' size='340' side='right'caption='[[1tkl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1tkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKL FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Coproporphyrinogen_oxidase Coproporphyrinogen oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.3 1.3.3.3]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= HEM13, YDR044W, YD5112.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkl OCA], [https://pdbe.org/1tkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tkl RCSB], [https://www.ebi.ac.uk/pdbsum/1tkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tkl ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Yeast Oxygen-Dependent Coproporphyrinogen Oxidase'''
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[https://www.uniprot.org/uniprot/HEM6_YEAST HEM6_YEAST] Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Coproporphyrinogen oxidase (CPO) is an essential enzyme that catalyzes the sixth step of the heme biosynthetic pathway. Unusually for heme biosynthetic enzymes, CPO exists in two evolutionarily and mechanistically distinct families, with eukaryotes and some prokaryotes employing members of the highly conserved oxygen-dependent CPO family. Here, we report the crystal structure of the oxygen-dependent CPO from Saccharomyces cerevisiae (Hem13p), which was determined by optimized sulfur anomalous scattering and refined to a resolution of 2.0 A. The protein adopts a novel structure that is quite different from predicted models and features a central flat seven-stranded anti-parallel sheet that is flanked by helices. The dimeric assembly, which is seen in different crystal forms, is formed by packing of helices and a short isolated strand that forms a beta-ladder with its counterpart in the partner subunit. The deep active-site cleft is lined by conserved residues and has been captured in open and closed conformations in two different crystal forms. A substratesized cavity is completely buried in the closed conformation by the approximately 8-A movement of a helix that forms a lid over the active site. The structure therefore suggests residues that likely play critical roles in catalysis and explains the deleterious effect of many of the mutations associated with the disease hereditary coproporphyria.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tkl_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1TKL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKL OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tkl ConSurf].
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Crystal structure of the oxygen-dependant coproporphyrinogen oxidase (Hem13p) of Saccharomyces cerevisiae., Phillips JD, Whitby FG, Warby CA, Labbe P, Yang C, Pflugrath JW, Ferrara JD, Robinson H, Kushner JP, Hill CP, J Biol Chem. 2004 Sep 10;279(37):38960-8. Epub 2004 Jun 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15194705 15194705]
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<div style="clear:both"></div>
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[[Category: Coproporphyrinogen oxidase]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Ferrara JD]]
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[[Category: Ferrara, J D.]]
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[[Category: Hill CP]]
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[[Category: Hill, C P.]]
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[[Category: Kushner JP]]
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[[Category: Kushner, J P.]]
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[[Category: Labbe P]]
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[[Category: Labbe, P.]]
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[[Category: Pflugrath JW]]
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[[Category: Pflugrath, J W.]]
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[[Category: Phillips JD]]
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[[Category: Phillips, J D.]]
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[[Category: Robinson H]]
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[[Category: Robinson, H.]]
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[[Category: Warby CA]]
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[[Category: Warby, C A.]]
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[[Category: Whitby FG]]
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[[Category: Whitby, F G.]]
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[[Category: Yang C]]
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[[Category: Yang, C.]]
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[[Category: coproporphyrinogen oxidase]]
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[[Category: enzyme]]
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[[Category: heme biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:19:36 2008''
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Current revision

Yeast Oxygen-Dependent Coproporphyrinogen Oxidase

PDB ID 1tkl

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