1tro

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[[Image:1tro.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION==
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|PDB= 1tro |SIZE=350|CAPTION= <scene name='initialview01'>1tro</scene>, resolution 1.900&Aring;
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<StructureSection load='1tro' size='340' side='right'caption='[[1tro]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>
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<table><tr><td colspan='2'>[[1tro]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TRO FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tro OCA], [https://pdbe.org/1tro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tro RCSB], [https://www.ebi.ac.uk/pdbsum/1tro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tro ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION'''
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== Function ==
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[https://www.uniprot.org/uniprot/TRPR_ECOLI TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tr/1tro_consurf.spt"</scriptWhenChecked>
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1TRO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRO OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of trp repressor/operator complex at atomic resolution., Otwinowski Z, Schevitz RW, Zhang RG, Lawson CL, Joachimiak A, Marmorstein RQ, Luisi BF, Sigler PB, Nature. 1988 Sep 22;335(6188):321-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3419502 3419502]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tro ConSurf].
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[[Category: Escherichia coli]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Joachimiak, A J.]]
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</StructureSection>
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[[Category: Lawson, C L.]]
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Luisi, B F.]]
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[[Category: Large Structures]]
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[[Category: Marmorstein, R.]]
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[[Category: Joachimiak A]]
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[[Category: Otwinowski, Z.]]
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[[Category: Lawson CL]]
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[[Category: Schevitz, R W.]]
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[[Category: Luisi BF]]
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[[Category: Sigler, P B.]]
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[[Category: Marmorstein R]]
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[[Category: Zhang, R G.]]
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[[Category: Otwinowski Z]]
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[[Category: TRP]]
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[[Category: Schevitz RW]]
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[[Category: double helix]]
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[[Category: Sigler PB]]
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[[Category: protein-dna complex]]
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[[Category: Zhang R-G]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:22:16 2008''
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Current revision

CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION

PDB ID 1tro

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