1u26

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[[Image:1u26.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate==
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|PDB= 1u26 |SIZE=350|CAPTION= <scene name='initialview01'>1u26</scene>, resolution 2.5&Aring;
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<StructureSection load='1u26' size='340' side='right'caption='[[1u26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>
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<table><tr><td colspan='2'>[[1u26]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U26 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u26 OCA], [https://pdbe.org/1u26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u26 RCSB], [https://www.ebi.ac.uk/pdbsum/1u26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u26 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7WUJ1_SELRU Q7WUJ1_SELRU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u2/1u26_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u26 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate'''
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==See Also==
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*[[Phytase 3D structures|Phytase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Various inositide phosphatases participate in the regulation of inositol polyphosphate signaling molecules. Plant phytases are phosphatases that hydrolyze phytate to less-phosphorylated myo-inositol derivatives and phosphate. The phytase from Selenomonas ruminantium shares no sequence homology with other microbial phytases. Its crystal structure revealed a phytase fold of the dual-specificity phosphatase type. The active site is located near a conserved cysteine-containing (Cys241) P loop. We also solved two other crystal forms in which an inhibitor, myo-inositol hexasulfate, is cocrystallized with the enzyme. In the "standby" and the "inhibited" crystal forms, the inhibitor is bound, respectively, in a pocket slightly away from Cys241 and at the substrate binding site where the phosphate group to be hydrolyzed is held close to the -SH group of Cys241. Our structural and mutagenesis studies allow us to visualize the way in which the P loop-containing phytase attracts and hydrolyzes the substrate (phytate) sequentially.
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[[Category: Large Structures]]
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==About this Structure==
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1U26 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U26 OCA].
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==Reference==
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Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15530366 15530366]
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[[Category: 5-phytase]]
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[[Category: Selenomonas ruminantium]]
[[Category: Selenomonas ruminantium]]
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[[Category: Single protein]]
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[[Category: Cheng KJ]]
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[[Category: Cheng, K J.]]
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[[Category: Chou CC]]
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[[Category: Chou, C C.]]
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[[Category: Chu HM]]
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[[Category: Chu, H M.]]
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[[Category: Guo RT]]
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[[Category: Guo, R T.]]
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[[Category: Lai HL]]
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[[Category: Lai, H L.]]
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[[Category: Lin TW]]
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[[Category: Lin, T W.]]
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[[Category: Selinger BL]]
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[[Category: Selinger, B L.]]
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[[Category: Shr HL]]
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[[Category: Shr, H L.]]
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[[Category: Tang TY]]
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[[Category: Tang, T Y.]]
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[[Category: Wang AH-J]]
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[[Category: Wang, A H.J.]]
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[[Category: IHS]]
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[[Category: p-loop]]
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[[Category: phytase]]
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[[Category: ptp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:22 2008''
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Current revision

Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate

PDB ID 1u26

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