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1u9p

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[[Image:1u9p.gif|left|200px]]
 
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{{Structure
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==Permuted single-chain Arc==
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|PDB= 1u9p |SIZE=350|CAPTION= <scene name='initialview01'>1u9p</scene>, resolution 1.9&Aring;
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<StructureSection load='1u9p' size='340' side='right'caption='[[1u9p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1u9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9P FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9p OCA], [https://pdbe.org/1u9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9p RCSB], [https://www.ebi.ac.uk/pdbsum/1u9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9p ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Permuted single-chain Arc'''
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[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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We designed a single-chain variant of the Arc repressor homodimer in which the beta strands that contact operator DNA are connected by a hairpin turn and the alpha helices that form the tetrahelical scaffold of the dimer are attached by a short linker. The designed protein represents a noncyclic permutation of secondary structural elements in another single-chain Arc molecule (Arc-L1-Arc), in which the two subunits are fused by a single linker. The permuted protein binds operator DNA with nanomolar affinity, refolds on the sub-millisecond time scale, and is as stable as Arc-L1-Arc. The crystal structure of the permuted protein reveals an essentially wild-type fold, demonstrating that crucial folding information is not encoded in the wild-type order of secondary structure. Noncyclic rearrangement of secondary structure may allow grouping of critical active-site residues in other proteins and could be a useful tool for protein design and minimization.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u9p_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1U9P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9P OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u9p ConSurf].
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Consolidating critical binding determinants by noncyclic rearrangement of protein secondary structure., Tabtiang RK, Cezairliyan BO, Grant RA, Cochrane JC, Sauer RT, Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2305-9. Epub 2005 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15689399 15689399]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Cezairliyan, B O.]]
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[[Category: Cezairliyan BO]]
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[[Category: Cochrane, J C.]]
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[[Category: Cochrane JC]]
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[[Category: Grant, R A.]]
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[[Category: Grant RA]]
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[[Category: Sauer, R T.]]
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[[Category: Sauer RT]]
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[[Category: Tabtiang, R K.]]
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[[Category: Tabtiang RK]]
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[[Category: arc]]
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[[Category: parc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:29:09 2008''
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Current revision

Permuted single-chain Arc

PDB ID 1u9p

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