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1v8q

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[[Image:1v8q.gif|left|200px]]
 
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{{Structure
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==Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8==
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|PDB= 1v8q |SIZE=350|CAPTION= <scene name='initialview01'>1v8q</scene>, resolution 2.80&Aring;
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<StructureSection load='1v8q' size='340' side='right'caption='[[1v8q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>
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<table><tr><td colspan='2'>[[1v8q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V8Q FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v8q OCA], [https://pdbe.org/1v8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v8q RCSB], [https://www.ebi.ac.uk/pdbsum/1v8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v8q ProSAT], [https://www.topsan.org/Proteins/RSGI/1v8q TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL27_THET8 RL27_THET8] Found on the solvent side of the large subunit.[HAMAP-Rule:MF_00539]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v8/1v8q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v8q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribosomal protein L27 is located near the peptidyltransferase center at the interface of ribosomal subunits, and is important for ribosomal assembly and function. We report the crystal structure of ribosomal protein L27 from Thermus thermophilus HB8, which was determined by the multiwavelength anomalous dispersion method and refined to an R-factor of 19.7% (R(free) = 23.6%) at 2.8 A resolution. The overall fold is an all beta-sheet hybrid. It consists of two sets of four-stranded beta-sheets formed around a well-defined hydrophobic core, with a highly positive charge on the protein surface. The structure of ribosomal protein L27 from T. thermophilus HB8 in the RNA-free form is investigated, and its functional roles in the ribosomal subunit are discussed.
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'''Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8'''
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Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8.,Wang H, Takemoto CH, Murayama K, Sakai H, Tatsuguchi A, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S Protein Sci. 2004 Oct;13(10):2806-10. Epub 2004 Aug 31. PMID:15340170<ref>PMID:15340170</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1v8q" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Ribosomal protein L27 is located near the peptidyltransferase center at the interface of ribosomal subunits, and is important for ribosomal assembly and function. We report the crystal structure of ribosomal protein L27 from Thermus thermophilus HB8, which was determined by the multiwavelength anomalous dispersion method and refined to an R-factor of 19.7% (R(free) = 23.6%) at 2.8 A resolution. The overall fold is an all beta-sheet hybrid. It consists of two sets of four-stranded beta-sheets formed around a well-defined hydrophobic core, with a highly positive charge on the protein surface. The structure of ribosomal protein L27 from T. thermophilus HB8 in the RNA-free form is investigated, and its functional roles in the ribosomal subunit are discussed.
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*[[Ribosomal protein L27|Ribosomal protein L27]]
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== References ==
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==About this Structure==
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<references/>
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1V8Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V8Q OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8., Wang H, Takemoto CH, Murayama K, Sakai H, Tatsuguchi A, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S, Protein Sci. 2004 Oct;13(10):2806-10. Epub 2004 Aug 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15340170 15340170]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Kuramitsu, S.]]
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[[Category: Kuramitsu S]]
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[[Category: Murayama, K.]]
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[[Category: Murayama K]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Shirouzu M]]
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[[Category: Shirouzu, M.]]
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[[Category: Takemoto-Hori C]]
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[[Category: Takemoto-Hori, C.]]
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[[Category: Terada T]]
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[[Category: Terada, T.]]
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[[Category: Wang H]]
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[[Category: Wang, H.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: DTT]]
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[[Category: proteomic]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:42:18 2008''
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Current revision

Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8

PDB ID 1v8q

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