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| ==PROTEASE INHIBITOR== | | ==PROTEASE INHIBITOR== |
- | <StructureSection load='1skz' size='340' side='right' caption='[[1skz]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='1skz' size='340' side='right'caption='[[1skz]], [[Resolution|resolution]] 1.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[1skz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haementeria_officinalis Haementeria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SKZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1skz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haementeria_officinalis Haementeria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKZ FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1skz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1skz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1skz RCSB], [http://www.ebi.ac.uk/pdbsum/1skz PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1skz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1skz OCA], [https://pdbe.org/1skz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1skz RCSB], [https://www.ebi.ac.uk/pdbsum/1skz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1skz ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/ANTA_HAEOF ANTA_HAEOF]] This highly disulfide-bonded protein is a potent inhibitor of factor Xa. May have therapeutic utility as an anticoagulant. Also exhibits a strong metastatic activity. | + | [https://www.uniprot.org/uniprot/ANTA_HAEOF ANTA_HAEOF] This highly disulfide-bonded protein is a potent inhibitor of factor Xa. May have therapeutic utility as an anticoagulant. Also exhibits a strong metastatic activity. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| </div> | | </div> |
| + | <div class="pdbe-citations 1skz" style="background-color:#fffaf0;"></div> |
| == References == | | == References == |
| <references/> | | <references/> |
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| </StructureSection> | | </StructureSection> |
| [[Category: Haementeria officinalis]] | | [[Category: Haementeria officinalis]] |
- | [[Category: Dijkstra, B W]] | + | [[Category: Large Structures]] |
- | [[Category: Krengel, U]] | + | [[Category: Dijkstra BW]] |
- | [[Category: Antistasin]] | + | [[Category: Krengel U]] |
- | [[Category: Factor xa inhibitor]]
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- | [[Category: Serine protease inhibitor]]
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- | [[Category: Thrombosis]]
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| Structural highlights
Function
ANTA_HAEOF This highly disulfide-bonded protein is a potent inhibitor of factor Xa. May have therapeutic utility as an anticoagulant. Also exhibits a strong metastatic activity.
Publication Abstract from PubMed
The three-dimensional structure of antistasin, a potent inhibitor of blood coagulation factor Xa, from the Mexican leech Haementeria officinalis was determined at 1.9 A resolution by X-ray crystallography. The structure reveals a novel protein fold composed of two homologous domains, each resembling the structure of hirustasin, a related 55-residue protease inhibitor. However, hirustasin has a different overall shape than the individual antistasin domains, it contains four rather than two beta-strands, and does not inhibit factor Xa. The two antistasin domains can be subdivided into two similarly sized subdomains with different relative orientations. Consequently, the domain shapes are different, the N-terminal domain being wedge-shaped and the C-terminal domain flat. Docking studies suggest that differences in domain shape enable the N-terminal, but not C-terminal, domain of antistasin to bind and inhibit factor Xa, even though both have a very similar reactive site. Furthermore, a putative exosite binding region could be defined in the N-terminal domain of antistasin, comprising residues 15-17, which is likely to interact with a cluster of positively charged residues on the factor Xa surface (Arg222/Lys223/Lys224). This exosite binding region explains the specificity and inhibitory potency of antistasin towards factor Xa. In the C-terminal domain of antistasin, these exosite interactions are prevented due to the different overall shape of this domain.
X-ray structure of antistasin at 1.9 A resolution and its modelled complex with blood coagulation factor Xa.,Lapatto R, Krengel U, Schreuder HA, Arkema A, de Boer B, Kalk KH, Hol WG, Grootenhuis PD, Mulders JW, Dijkema R, Theunissen HJ, Dijkstra BW EMBO J. 1997 Sep 1;16(17):5151-61. PMID:9311976[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lapatto R, Krengel U, Schreuder HA, Arkema A, de Boer B, Kalk KH, Hol WG, Grootenhuis PD, Mulders JW, Dijkema R, Theunissen HJ, Dijkstra BW. X-ray structure of antistasin at 1.9 A resolution and its modelled complex with blood coagulation factor Xa. EMBO J. 1997 Sep 1;16(17):5151-61. PMID:9311976 doi:10.1093/emboj/16.17.5151
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