1w0i

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[[Image:1w0i.gif|left|200px]]
 
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{{Structure
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==Arabidopsis thaliana Mitochondrial KAS==
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|PDB= 1w0i |SIZE=350|CAPTION= <scene name='initialview01'>1w0i</scene>, resolution 2.10&Aring;
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<StructureSection load='1w0i' size='340' side='right'caption='[[1w0i]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE= <scene name='pdbsite=KAA:So4+Binding+Site+For+Chain+B'>KAA</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1w0i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W0I FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w0i OCA], [https://pdbe.org/1w0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w0i RCSB], [https://www.ebi.ac.uk/pdbsum/1w0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w0i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KASM_ARATH KASM_ARATH] Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins.<ref>PMID:17616510</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w0/1w0i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w0i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzyme accomplishes the vital condensation steps in constructing fatty acid carbon skeletons. The product profile of mtKAS is unusual in that C8 and C(14-16) fatty acyl chains predominate. An enzyme architecture that likely is the basis for the observed bimodal profile of mtKAS products can be derived from the shape of the acyl binding pocket.
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'''ARABIDOPSIS THALIANA MITOCHONDRIAL KAS'''
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Structure of the mitochondrial beta-ketoacyl-[acyl carrier protein] synthase from Arabidopsis and its role in fatty acid synthesis.,Olsen JG, Rasmussen AV, von Wettstein-Knowles P, Henriksen A FEBS Lett. 2004 Nov 5;577(1-2):170-4. PMID:15527780<ref>PMID:15527780</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1w0i" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzyme accomplishes the vital condensation steps in constructing fatty acid carbon skeletons. The product profile of mtKAS is unusual in that C8 and C(14-16) fatty acyl chains predominate. An enzyme architecture that likely is the basis for the observed bimodal profile of mtKAS products can be derived from the shape of the acyl binding pocket.
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*[[Acyl carrier protein synthase 3D structures|Acyl carrier protein synthase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1W0I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0I OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structure of the mitochondrial beta-ketoacyl-[acyl carrier protein] synthase from Arabidopsis and its role in fatty acid synthesis., Olsen JG, Rasmussen AV, von Wettstein-Knowles P, Henriksen A, FEBS Lett. 2004 Nov 5;577(1-2):170-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15527780 15527780]
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Henriksen A]]
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[[Category: Henriksen, A.]]
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[[Category: Olsen JG]]
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[[Category: Olsen, J G.]]
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[[Category: Rasmussen AV]]
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[[Category: Rasmussen, A V.]]
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[[Category: Von Wettstein-Knowles P]]
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[[Category: Wettstein-Knowles, P Von.]]
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[[Category: K]]
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[[Category: SO4]]
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[[Category: beta-ketoacyl-(acyl carrier protein) synthase]]
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[[Category: condensing enzyme]]
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[[Category: fatty acid elongation]]
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[[Category: lipid metabolism]]
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[[Category: synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:27 2008''
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Current revision

Arabidopsis thaliana Mitochondrial KAS

PDB ID 1w0i

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