4arz

From Proteopedia

(Difference between revisions)

Current revision

The crystal structure of Gtr1p-Gtr2p complexed with GTP-GDP

Structural highlights

4arz is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.1Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GTR2_YEAST GTPase. Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.^[1] ^[2]

Publication Abstract from PubMed

The heterodimeric Rag GTPases consisting of RagA (or RagB) and RagC (or RagD) are the key regulator activating the target of rapamycin complex 1 (TORC1) in response to the level of amino acids. The heterodimer between GTP-loaded RagA/B and GDP-loaded RagC/D is the most active form that binds Raptor and leads to the activation of TORC1. Here, we present the crystal structure of Gtr1p(GTP)-Gtr2p(GDP), the active yeast Rag GTPase heterodimer. The structure reveals that GTP-to-GDP conversion on Gtr2p results in a large conformational transition of this subunit, including a large scale rearrangement of a long segment whose corresponding region in RagA is involved in binding to Raptor. In addition, the two GTPase domains of the heterodimer are brought to contact with each other, but without causing any conformational change of the Gtr1p subunit. These features explain how the nucleotide-bound statuses of the two GTPases subunits switch the Raptor binding affinity on and off.

Crystal Structure of the Gtr1pGTP-Gtr2pGDP Protein Complex Reveals Large Structural Rearrangements Triggered by GTP-to-GDP Conversion.,Jeong JH, Lee KH, Kim YM, Kim DH, Oh BH, Kim YG J Biol Chem. 2012 Aug 24;287(35):29648-53. Epub 2012 Jul 17. PMID:22807443^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dubouloz F, Deloche O, Wanke V, Cameroni E, De Virgilio C. The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol Cell. 2005 Jul 1;19(1):15-26. PMID:15989961 doi:10.1016/j.molcel.2005.05.020
↑ Gao M, Kaiser CA. A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol. 2006 Jul;8(7):657-67. Epub 2006 May 28. PMID:16732272 doi:ncb1419
↑ Jeong JH, Lee KH, Kim YM, Kim DH, Oh BH, Kim YG. Crystal Structure of the Gtr1pGTP-Gtr2pGDP Protein Complex Reveals Large Structural Rearrangements Triggered by GTP-to-GDP Conversion. J Biol Chem. 2012 Aug 24;287(35):29648-53. Epub 2012 Jul 17. PMID:22807443 doi:http://dx.doi.org/10.1074/jbc.C112.384420

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4arz"

Categories: Large Structures | Saccharomyces cerevisiae | Jeong JH | Kim YG

@@ Line 1: / Line 1: @@
 ==The crystal structure of Gtr1p-Gtr2p complexed with GTP-GDP==
-<StructureSection load='4arz' size='340' side='right' caption='[[4arz]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+<StructureSection load='4arz' size='340' side='right'caption='[[4arz]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4arz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4arz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4arz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4arz RCSB], [http://www.ebi.ac.uk/pdbsum/4arz PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4arz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arz OCA], [https://pdbe.org/4arz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4arz RCSB], [https://www.ebi.ac.uk/pdbsum/4arz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4arz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GTR1_YEAST GTR1_YEAST]] GTPase component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Functionally associated with the inorganic phosphate transporter PHO84. It is probably required for modulation of PHO84 or involved in its intracellular localization. [[http://www.uniprot.org/uniprot/GTR2_YEAST GTR2_YEAST]] GTPase. Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.<ref>PMID:15989961</ref> <ref>PMID:16732272</ref>
+[https://www.uniprot.org/uniprot/GTR2_YEAST GTR2_YEAST] GTPase. Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.<ref>PMID:15989961</ref> <ref>PMID:16732272</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4arz" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[GTP-binding protein|GTP-binding protein]]
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Jeong, J H]]
+[[Category: Jeong JH]]
-[[Category: Kim, Y G]]
+[[Category: Kim YG]]
-[[Category: Cell growth]]
-[[Category: Gtpase]]
-[[Category: Hydrolase]]

4arz

From Proteopedia

Current revision

The crystal structure of Gtr1p-Gtr2p complexed with GTP-GDP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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