4r5i

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==Crystal structure of the DnaK C-terminus with the substrate peptide NRLLLTG==
==Crystal structure of the DnaK C-terminus with the substrate peptide NRLLLTG==
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<StructureSection load='4r5i' size='340' side='right' caption='[[4r5i]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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<StructureSection load='4r5i' size='340' side='right'caption='[[4r5i]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4r5i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R5I FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4r5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R5I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9702&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r5i RCSB], [http://www.ebi.ac.uk/pdbsum/4r5i PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r5i OCA], [https://pdbe.org/4r5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r5i RCSB], [https://www.ebi.ac.uk/pdbsum/4r5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r5i ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/DNAK_ECOLI DNAK_ECOLI]] Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.[HAMAP-Rule:MF_00332]
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[https://www.uniprot.org/uniprot/DNAK_ECOLI DNAK_ECOLI] Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.[HAMAP-Rule:MF_00332]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The stress-inducible mammalian heat shock protein 70 (HSP70) and its bacterial orthologue DnaK are highly conserved nucleotide binding molecular chaperones. They represent critical regulators of cellular proteostasis, especially during conditions of enhanced stress. Cancer cells rely on HSP70 for survival, and this chaperone represents an attractive new therapeutic target. We have used a structure-activity approach and biophysical methods to characterize a class of inhibitors that bind to a unique allosteric site within the C-terminus of HSP70 and DnaK. Data from X-ray crystallography together with isothermal titration calorimetry, mutagenesis, and cell-based assays indicate that these inhibitors bind to a previously unappreciated allosteric pocket formed within the non-ATP-bound protein state. Moreover, binding of inhibitor alters the local protein conformation, resulting in reduced chaperone-client interactions and impairment of proteostasis. Our findings thereby provide a new chemical scaffold and target platform for both HSP70 and DnaK; these will be important tools with which to interrogate chaperone function and to aid ongoing efforts to optimize potency and efficacy in developing modulators of these chaperones for therapeutic use.
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Structural Basis for the Inhibition of HSP70 and DnaK Chaperones by Small-Molecule Targeting of a C-Terminal Allosteric Pocket.,Leu JI, Zhang P, Murphy ME, Marmorstein R, George DL ACS Chem Biol. 2014 Aug 28. PMID:25148104<ref>PMID:25148104</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Heat Shock Protein structures|Heat Shock Protein structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: George, D L]]
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[[Category: Escherichia coli K-12]]
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[[Category: Leu, J I]]
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[[Category: Large Structures]]
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[[Category: Marmorstein, R]]
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[[Category: Synthetic construct]]
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[[Category: Murphy, M E]]
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[[Category: George DL]]
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[[Category: Zhang, P]]
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[[Category: Leu JI]]
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[[Category: Beta sheet]]
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[[Category: Marmorstein R]]
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[[Category: Chaperone]]
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[[Category: Murphy ME]]
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[[Category: Helical bundle]]
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[[Category: Zhang P]]
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[[Category: Membrane]]
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[[Category: Substrate binding]]
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Current revision

Crystal structure of the DnaK C-terminus with the substrate peptide NRLLLTG

PDB ID 4r5i

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