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4d5y

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'''Unreleased structure'''
 
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The entry 4d5y is ON HOLD until sometime in the future
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==Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state==
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<SX load='4d5y' size='340' side='right' viewer='molstar' caption='[[4d5y]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4d5y]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D5Y FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5y OCA], [https://pdbe.org/4d5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d5y RCSB], [https://www.ebi.ac.uk/pdbsum/4d5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/G1TT27_RABIT G1TT27_RABIT]]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cricket paralysis virus (CrPV) uses an internal ribosomal entry site (IRES) to hijack the ribosome. In a remarkable RNA-based mechanism involving neither initiation factor nor initiator tRNA, the CrPV IRES jumpstarts translation in the elongation phase from the ribosomal A site. Here, we present cryoelectron microscopy (cryo-EM) maps of 80SCrPV-STOPeRF1eRF3GMPPNP and 80SCrPV-STOPeRF1 complexes, revealing a previously unseen binding state of the IRES and directly rationalizing that an eEF2-dependent translocation of the IRES is required to allow the first A-site occupation. During this unusual translocation event, the IRES undergoes a pronounced conformational change to a more stretched conformation. At the same time, our structural analysis provides information about the binding modes of eRF1eRF3GMPPNP and eRF1 in a minimal system. It shows that neither eRF3 nor ABCE1 are required for the active conformation of eRF1 at the intersection between eukaryotic termination and recycling.
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Authors: Muhs, M., Hilal, T., Mielke, T., Skabkin, M.A., Sanbonmatsu, K.Y., Pestova, T.V., Spahn, C.M.T.
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Cryo-EM of Ribosomal 80S Complexes with Termination Factors Reveals the Translocated Cricket Paralysis Virus IRES.,Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM Mol Cell. 2015 Feb 5;57(3):422-432. doi: 10.1016/j.molcel.2014.12.016. Epub 2015 , Jan 15. PMID:25601755<ref>PMID:25601755</ref>
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Description: Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Sanbonmatsu, K.Y]]
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<div class="pdbe-citations 4d5y" style="background-color:#fffaf0;"></div>
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[[Category: Skabkin, M.A]]
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== References ==
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[[Category: Muhs, M]]
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<references/>
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[[Category: Pestova, T.V]]
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__TOC__
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[[Category: Mielke, T]]
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</SX>
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[[Category: Hilal, T]]
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[[Category: Large Structures]]
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[[Category: Spahn, C.M.T]]
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[[Category: Oryctolagus cuniculus]]
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[[Category: Hilal T]]
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[[Category: Mielke T]]
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[[Category: Muhs M]]
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[[Category: Pestova TV]]
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[[Category: Sanbonmatsu KY]]
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[[Category: Skabkin MA]]
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[[Category: Spahn CMT]]

Current revision

Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

4d5y, resolution 9.00Å

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