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1xt6

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[[Image:1xt6.gif|left|200px]]
 
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{{Structure
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==S35C Flavodoxin Mutant in the semiquinone state==
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|PDB= 1xt6 |SIZE=350|CAPTION= <scene name='initialview01'>1xt6</scene>, resolution 1.80&Aring;
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<StructureSection load='1xt6' size='340' side='right'caption='[[1xt6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN MONONUCLEOTIDE'>FMN</scene>
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<table><tr><td colspan='2'>[[1xt6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XT6 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xt6 OCA], [https://pdbe.org/1xt6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xt6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xt6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xt6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH] Low-potential electron donor to a number of redox enzymes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xt/1xt6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xt6 ConSurf].
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<div style="clear:both"></div>
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'''S35C Flavodoxin Mutant in the semiquinone state'''
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==See Also==
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*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
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[[Category: Desulfovibrio vulgaris str. Hildenborough]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Artali R]]
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1XT6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].
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[[Category: Bombieri G]]
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[[Category: Cassetta A]]
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==Reference==
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[[Category: Cavazzini D]]
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Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state., Artali R, Marchini N, Meneghetti F, Cavazzini D, Cassetta A, Sassone C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):481-4. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805604 15805604]
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[[Category: Gilardi G]]
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[[Category: Desulfovibrio vulgaris]]
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[[Category: Marchini N]]
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[[Category: Single protein]]
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[[Category: Meneghetti F]]
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[[Category: Artali, R.]]
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[[Category: Rossi GL]]
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[[Category: Bombieri, G.]]
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[[Category: Sassone C]]
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[[Category: Cassetta, A.]]
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[[Category: Cavazzini, D.]]
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[[Category: Gilardi, G.]]
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[[Category: Marchini, N.]]
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[[Category: Meneghetti, F.]]
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[[Category: Rossi, G L.]]
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[[Category: Sassone, C.]]
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[[Category: FMN]]
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[[Category: flavodoxin]]
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[[Category: mutant]]
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[[Category: protein]]
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[[Category: s35c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:14:25 2008''
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Current revision

S35C Flavodoxin Mutant in the semiquinone state

PDB ID 1xt6

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