1y10

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[[Image:1y10.gif|left|200px]]
 
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{{Structure
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==Mycobacterial adenylyl cyclase Rv1264, holoenzyme, inhibited state==
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|PDB= 1y10 |SIZE=350|CAPTION= <scene name='initialview01'>1y10</scene>, resolution 2.30&Aring;
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<StructureSection load='1y10' size='340' side='right'caption='[[1y10]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=1PE:PENTAETHYLENE GLYCOL'>1PE</scene>
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<table><tr><td colspan='2'>[[1y10]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y10 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y10 OCA], [https://pdbe.org/1y10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y10 RCSB], [https://www.ebi.ac.uk/pdbsum/1y10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y10 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU] Catalyzes the formation of the second messenger cAMP.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y1/1y10_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y10 ConSurf].
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<div style="clear:both"></div>
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'''Mycobacterial adenylyl cyclase Rv1264, holoenzyme, inhibited state'''
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==See Also==
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*[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Class III adenylyl cyclases contain catalytic and regulatory domains, yet structural insight into their interactions is missing. We show that the mycobacterial adenylyl cyclase Rv1264 is rendered a pH sensor by its N-terminal domain. In the structure of the inhibited state, catalytic and regulatory domains share a large interface involving catalytic residues. In the structure of the active state, the two catalytic domains rotate by 55 degrees to form two catalytic sites at their interface. Two alpha helices serve as molecular switches. Mutagenesis is consistent with a regulatory role of the structural transition, and we suggest that the transition is regulated by pH.
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[[Category: Large Structures]]
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==About this Structure==
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1Y10 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y10 OCA].
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==Reference==
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The structure of a pH-sensing mycobacterial adenylyl cyclase holoenzyme., Tews I, Findeisen F, Sinning I, Schultz A, Schultz JE, Linder JU, Science. 2005 May 13;308(5724):1020-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15890882 15890882]
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[[Category: Adenylate cyclase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Findeisen F]]
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[[Category: Findeisen, F.]]
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[[Category: Linder JU]]
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[[Category: Linder, J U.]]
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[[Category: Schultz A]]
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[[Category: Schultz, A.]]
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[[Category: Schultz JE]]
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[[Category: Schultz, J E.]]
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[[Category: Sinning I]]
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[[Category: Sinning, I.]]
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[[Category: Tews I]]
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[[Category: Tews, I.]]
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[[Category: 1PE]]
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[[Category: CA]]
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[[Category: adenylyl cyclase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:05 2008''
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Current revision

Mycobacterial adenylyl cyclase Rv1264, holoenzyme, inhibited state

PDB ID 1y10

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