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| | ==Crystal structure of telomere capping protein Stn1 from Saccharomyces cerevisiae== | | ==Crystal structure of telomere capping protein Stn1 from Saccharomyces cerevisiae== |
| - | <StructureSection load='3k10' size='340' side='right' caption='[[3k10]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3k10' size='340' side='right'caption='[[3k10]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k10]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K10 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K10 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k10]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K10 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k0x|3k0x]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D4456, STN1, YD8554.15, YDR082W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k10 OCA], [https://pdbe.org/3k10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k10 RCSB], [https://www.ebi.ac.uk/pdbsum/3k10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k10 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k10 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k10 RCSB], [http://www.ebi.ac.uk/pdbsum/3k10 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/STN1_YEAST STN1_YEAST]] Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13.<ref>PMID:9042864</ref> <ref>PMID:11230140</ref> | + | [https://www.uniprot.org/uniprot/STN1_YEAST STN1_YEAST] Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13.<ref>PMID:9042864</ref> <ref>PMID:11230140</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3k10" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Batey, R T]] | + | [[Category: Batey RT]] |
| - | [[Category: Gelinas, A D]] | + | [[Category: Gelinas AD]] |
| - | [[Category: Reyes, F E]] | + | [[Category: Reyes FE]] |
| - | [[Category: Wuttke, D S]] | + | [[Category: Wuttke DS]] |
| - | [[Category: Chromosomal protein]]
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| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Telomere]]
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| - | [[Category: Telomere capping]]
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| - | [[Category: Winged helix turn helix]]
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| Structural highlights
Function
STN1_YEAST Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13.[1] [2]
Publication Abstract from PubMed
Telomeres must be capped to preserve chromosomal stability. The conserved Stn1 and Ten1 proteins are required for proper capping of the telomere, although the mechanistic details of how they contribute to telomere maintenance are unclear. Here, we report the crystal structures of the C-terminal domain of the Saccharomyces cerevisiae Stn1 and the Schizosaccharomyces pombe Ten1 proteins. These structures reveal striking similarities to corresponding subunits in the replication protein A complex, further supporting an evolutionary link between telomere maintenance proteins and DNA repair complexes. Our structural and in vivo data of Stn1 identify a new domain that has evolved to support a telomere-specific role in chromosome maintenance. These findings endorse a model of an evolutionarily conserved mechanism of DNA maintenance that has developed as a result of increased chromosomal structural complexity.
Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain.,Gelinas AD, Paschini M, Reyes FE, Heroux A, Batey RT, Lundblad V, Wuttke DS Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19298-303. Epub 2009 Nov 2. PMID:19884503[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grandin N, Reed SI, Charbonneau M. Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13. Genes Dev. 1997 Feb 15;11(4):512-27. PMID:9042864
- ↑ Grandin N, Damon C, Charbonneau M. Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13. EMBO J. 2001 Mar 1;20(5):1173-83. PMID:11230140 doi:10.1093/emboj/20.5.1173
- ↑ Gelinas AD, Paschini M, Reyes FE, Heroux A, Batey RT, Lundblad V, Wuttke DS. Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain. Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19298-303. Epub 2009 Nov 2. PMID:19884503
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