4v1y
From Proteopedia
(Difference between revisions)
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of the hexameric atrazine chlorohydrolase, AtzA== |
| + | <StructureSection load='4v1y' size='340' side='right'caption='[[4v1y]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4v1y]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V1Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v1y OCA], [https://pdbe.org/4v1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v1y RCSB], [https://www.ebi.ac.uk/pdbsum/4v1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v1y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ATZA_PSESD ATZA_PSESD] Hydrolytically dechlorinates atrazine to hydroxyatrazine. Dechlorinates also simazine, and desethylatrazine but is not active with melamine, terbutylazine, or desethyldesisopropylatrazine.<ref>PMID:8759853</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Atrazine chlorohydrolase (AtzA) was discovered and purified in the early 1990s from soil that had been exposed to the widely used herbicide atrazine. It was subsequently found that this enzyme catalyzes the first and necessary step in the breakdown of atrazine by the soil organism Pseudomonas sp. strain ADP. Although it has taken 20 years, a crystal structure of the full hexameric form of AtzA has now been obtained. AtzA is less well adapted to its physiological role (i.e. atrazine dechlorination) than the alternative metal-dependent atrazine chlorohydrolase (TrzN), with a substrate-binding pocket that is under considerable strain and for which the substrate is a poor fit. | ||
| - | + | The structure of the hexameric atrazine chlorohydrolase AtzA.,Peat TS, Newman J, Balotra S, Lucent D, Warden AC, Scott C Acta Crystallogr D Biol Crystallogr. 2015 Mar 1;71(Pt 3):710-20. doi:, 10.1107/S1399004715000619. Epub 2015 Feb 26. PMID:25760618<ref>PMID:25760618</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4v1y" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Newman | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Pseudomonas sp. ADP]] | ||
| + | [[Category: Balotra S]] | ||
| + | [[Category: Lucent D]] | ||
| + | [[Category: Newman J]] | ||
| + | [[Category: Peat TS]] | ||
| + | [[Category: Scott C]] | ||
| + | [[Category: Warden AC]] | ||
Current revision
The structure of the hexameric atrazine chlorohydrolase, AtzA
| |||||||||||
