4v3l

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'''Unreleased structure'''
 
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The entry 4v3l is ON HOLD
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==RNF38-UB-UbcH5B-Ub complex==
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<StructureSection load='4v3l' size='340' side='right'caption='[[4v3l]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4v3l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V3L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V3L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3l OCA], [https://pdbe.org/4v3l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v3l RCSB], [https://www.ebi.ac.uk/pdbsum/4v3l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v3l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UB2D2_HUMAN UB2D2_HUMAN] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.<ref>PMID:10329681</ref> <ref>PMID:15280377</ref> <ref>PMID:18042044</ref> <ref>PMID:18703417</ref> <ref>PMID:18359941</ref> <ref>PMID:19854139</ref> <ref>PMID:20403326</ref> <ref>PMID:20061386</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (UbB) enhances both RING-independent and RING-dependent UbcH5B-catalyzed donor Ub (UbD) transfer, but with a more prominent effect in RING-dependent transfer. UbB enhances RING E3s' affinities for UbcH5B-Ub, and RING E3-UbcH5B-Ub complex improves UbB's affinity for UbcH5B. A comparison of the crystal structures of a RING E3, RNF38, bound to UbcH5B-Ub in the absence and presence of UbB, together with molecular dynamics simulation and biochemical analyses, suggests UbB restricts the flexibility of UbcH5B's alpha1 and alpha1beta1 loop. UbB supports E3 function by stabilizing the RING E3-UbcH5B-Ub complex, thereby improving the catalytic efficiency of Ub transfer. Thus, UbB serves as an allosteric activator of RING E3-mediated Ub transfer.
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Authors: Buetow, L., Gabrielsen, M., Anthony, N.G., Dou, H., Patel, A., Aitkenhead, H., Sibbet, G.J., Smith, B.O., Huang, D.T.
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Activation of a Primed RING E3-E2-Ubiquitin Complex by Non-Covalent Ubiquitin.,Buetow L, Gabrielsen M, Anthony NG, Dou H, Patel A, Aitkenhead H, Sibbet GJ, Smith BO, Huang DT Mol Cell. 2015 Mar 18. pii: S1097-2765(15)00131-8. doi:, 10.1016/j.molcel.2015.02.017. PMID:25801170<ref>PMID:25801170</ref>
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Description: E3-E2-Ub
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Aitkenhead, H]]
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<div class="pdbe-citations 4v3l" style="background-color:#fffaf0;"></div>
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[[Category: Buetow, L]]
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[[Category: Smith, B.O]]
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==See Also==
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[[Category: Anthony, N.G]]
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*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
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[[Category: Dou, H]]
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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[[Category: Huang, D.T]]
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== References ==
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[[Category: Gabrielsen, M]]
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<references/>
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[[Category: Sibbet, G.J]]
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__TOC__
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[[Category: Patel, A]]
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Aitkenhead H]]
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[[Category: Anthony NG]]
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[[Category: Buetow L]]
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[[Category: Dou H]]
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[[Category: Gabrielsen M]]
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[[Category: Huang DT]]
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[[Category: Patel A]]
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[[Category: Sibbet GJ]]
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[[Category: Smith BO]]

Current revision

RNF38-UB-UbcH5B-Ub complex

PDB ID 4v3l

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