4g6d

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==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
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<StructureSection load='4g6d' size='340' side='right' caption='[[4g6d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='4g6d' size='340' side='right'caption='[[4g6d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4g6d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325] and [http://en.wikipedia.org/wiki/Staphylococcus_phage_g1 Staphylococcus phage g1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6D FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4g6d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325] and [https://en.wikipedia.org/wiki/Staphylococcus_virus_G1 Staphylococcus virus G1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6D FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoD, plaC, sigA, SAOUHSC_01662 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 Staphylococcus aureus subsp. aureus NCTC 8325])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9971&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6d RCSB], [http://www.ebi.ac.uk/pdbsum/4g6d PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6d OCA], [https://pdbe.org/4g6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6d RCSB], [https://www.ebi.ac.uk/pdbsum/4g6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RPOD_STAA8 RPOD_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
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[https://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) sigma(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-A-resolution crystal structure of the complex between gp67 and Sau sigma(A) domain 4 (sigma(A)(4)) explains how gp67 joins the RNAP promoter complex through sigma(A)(4) without significantly affecting sigma(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, sigma(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP alpha-subunit C-terminal domain (alphaCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (sigma(A)), and selectively affects the function of another subunit (alphaCTD) depending on promoter usage.
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Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.,Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120<ref>PMID:23178120</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Sigma factor|Sigma factor]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]
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[[Category: Large Structures]]
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[[Category: Staphylococcus phage g1]]
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[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
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[[Category: Darst, S A]]
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[[Category: Staphylococcus virus G1]]
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[[Category: Osmundson, J S]]
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[[Category: Darst SA]]
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[[Category: Dna binding protein]]
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[[Category: Osmundson JS]]
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[[Category: Staphylococcus aureus rna polymerase]]
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Current revision

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

PDB ID 4g6d

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