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4bxs
From Proteopedia
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==Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis== | ==Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis== | ||
| - | <StructureSection load='4bxs' size='340' side='right' caption='[[4bxs]], [[Resolution|resolution]] 3.32Å' scene=''> | + | <StructureSection load='4bxs' size='340' side='right'caption='[[4bxs]], [[Resolution|resolution]] 3.32Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4bxs]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[4bxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonaja_textilis Pseudonaja textilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand= | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.32Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxs OCA], [https://pdbe.org/4bxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxs RCSB], [https://www.ebi.ac.uk/pdbsum/4bxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxs ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/FAXC_PSETE FAXC_PSETE] Snake prothrombin activator that attacks the hemostatic system of prey. This non-catalytic subunit is functionally similar to blood coagulation factor V (PubMed:12362232, PubMed:23869089). It serves as a critical cofactor for the prothrombinase activity of the catalytic subunit, which is similar to the blood coagulation factor X (PubMed:12362232, PubMed:23869089). The complex converts prothrombin to thrombin by sequential cleavage at two positions, Arg-320 followed by Arg-271 (PubMed:23869089). Cleavage at Arg-320 produces an active intermediate known as meizothrombin (PubMed:23869089). Meizothrombin is the 'second' substrate for prothrombinase, and it docks in an altered manner to present the second cleavage site (271) (PubMed:23869089). Cleavage at Arg-271 releases active thrombin from its pro-fragment (PubMed:23869089). This order of events is reversed if the protease component of prothrombinase is used on its own, suggesting that the 271 site is inherently more accessible to proteolysis (PubMed:23869089). The complex converts prothrombin to thrombin in presence but also in the absence of membrane (PubMed:23869089).<ref>PMID:12362232</ref> <ref>PMID:23869089</ref> |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
| + | <div class="pdbe-citations 4bxs" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Pseudonaja textilis]] | [[Category: Pseudonaja textilis]] | ||
| - | [[Category: Adams | + | [[Category: Adams TE]] |
| - | [[Category: Camire | + | [[Category: Camire RM]] |
| - | [[Category: Huntington | + | [[Category: Huntington JA]] |
| - | [[Category: Johnson | + | [[Category: Johnson DJD]] |
| - | [[Category: Krishnaswamy | + | [[Category: Krishnaswamy S]] |
| - | [[Category: Lechtenberg | + | [[Category: Lechtenberg BC]] |
| - | [[Category: Murray-Rust | + | [[Category: Murray-Rust TA]] |
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Current revision
Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis
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