1zc5
From Proteopedia
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| - | [[Image:1zc5.gif|left|200px]] | ||
| - | + | ==Structure of the RNA signal essential for translational frameshifting in HIV-1== | |
| - | + | <StructureSection load='1zc5' size='340' side='right'caption='[[1zc5]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1zc5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZC5 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | | | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zc5 OCA], [https://pdbe.org/1zc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zc5 RCSB], [https://www.ebi.ac.uk/pdbsum/1zc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zc5 ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | ''' | + | == Publication Abstract from PubMed == |
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Many pathogenic viruses use a programmed -1 translational frameshifting mechanism to regulate synthesis of their structural and enzymatic proteins. Frameshifting is vital for viral replication. A slippery sequence bound at the ribosomal A and P sites as well as a downstream stimulatory RNA structure are essential for frameshifting. Conflicting data have been reported concerning the structure of the downstream RNA signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution structure of the HIV-1 frameshifting RNA signal was solved by heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold with an internal three-nucleotide bulge. The internal loop introduces a bend between the lower and upper helical regions, a structural feature often seen in frameshifting pseudoknots. The NMR structure correlates with chemical probing data. The upper stem rich in conserved G-C Watson-Crick base-pairs is highly stable, whereas the bulge region and the lower stem are more flexible. | Many pathogenic viruses use a programmed -1 translational frameshifting mechanism to regulate synthesis of their structural and enzymatic proteins. Frameshifting is vital for viral replication. A slippery sequence bound at the ribosomal A and P sites as well as a downstream stimulatory RNA structure are essential for frameshifting. Conflicting data have been reported concerning the structure of the downstream RNA signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution structure of the HIV-1 frameshifting RNA signal was solved by heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold with an internal three-nucleotide bulge. The internal loop introduces a bend between the lower and upper helical regions, a structural feature often seen in frameshifting pseudoknots. The NMR structure correlates with chemical probing data. The upper stem rich in conserved G-C Watson-Crick base-pairs is highly stable, whereas the bulge region and the lower stem are more flexible. | ||
| - | + | Structure of the RNA signal essential for translational frameshifting in HIV-1.,Gaudin C, Mazauric MH, Traikia M, Guittet E, Yoshizawa S, Fourmy D J Mol Biol. 2005 Jun 24;349(5):1024-35. PMID:15907937<ref>PMID:15907937</ref> | |
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| - | Structure of the RNA signal essential for translational frameshifting in HIV-1., Gaudin C, Mazauric MH, Traikia M, Guittet E, Yoshizawa S, Fourmy D | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 1zc5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fourmy D]] | ||
| + | [[Category: Gaudin C]] | ||
| + | [[Category: Guittet E]] | ||
| + | [[Category: Mazauric MH]] | ||
| + | [[Category: Traikia M]] | ||
| + | [[Category: Yoshizawa S]] | ||
Current revision
Structure of the RNA signal essential for translational frameshifting in HIV-1
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