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1zeq

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[[Image:1zeq.gif|left|200px]]
 
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{{Structure
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==1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli==
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|PDB= 1zeq |SIZE=350|CAPTION= <scene name='initialview01'>1zeq</scene>, resolution 1.50&Aring;
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<StructureSection load='1zeq' size='340' side='right'caption='[[1zeq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1zeq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZEQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE= cusF, cusX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zeq OCA], [https://pdbe.org/1zeq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zeq RCSB], [https://www.ebi.ac.uk/pdbsum/1zeq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zeq ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli'''
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[https://www.uniprot.org/uniprot/CUSF_ECOLI CUSF_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver. Binds one copper per polypeptide.<ref>PMID:11399769</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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We have determined the crystal structure of apo-CusF, a periplasmic protein involved in copper and silver resistance in Escherichia coli. The protein forms a five-stranded beta-barrel, classified as an OB-fold, which is a unique topology for a copper-binding protein. NMR chemical shift mapping experiments suggest that Cu(I) is bound by conserved residues H36, M47, and M49 located in beta-strands 2 and 3. These residues are clustered at one end of the beta-barrel, and their side chains are oriented toward the interior of the barrel. Cu(I) can be modeled into the apo-CusF structure with only minimal structural changes using H36, M47, and M49 as ligands. The unique structure and metal binding site of CusF are distinct from those of previously characterized copper-binding proteins.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/1zeq_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1ZEQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEQ OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zeq ConSurf].
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A novel copper-binding fold for the periplasmic copper resistance protein CusF., Loftin IR, Franke S, Roberts SA, Weichsel A, Heroux A, Montfort WR, Rensing C, McEvoy MM, Biochemistry. 2005 Aug 9;44(31):10533-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16060662 16060662]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Franke, S.]]
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[[Category: Franke S]]
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[[Category: Heroux, A.]]
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[[Category: Heroux A]]
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[[Category: Loftin, I R.]]
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[[Category: Loftin IR]]
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[[Category: McEvoy, M M.]]
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[[Category: McEvoy MM]]
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[[Category: Montfort, W R.]]
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[[Category: Montfort WR]]
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[[Category: Rensing, C.]]
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[[Category: Rensing C]]
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[[Category: Roberts, S A.]]
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[[Category: Roberts SA]]
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[[Category: Weichsel, A.]]
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[[Category: Weichsel A]]
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[[Category: beta barrel]]
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[[Category: copper-binding]]
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[[Category: metallochaperone]]
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[[Category: ob-fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:34:34 2008''
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Current revision

1.5 A Structure of apo-CusF residues 6-88 from Escherichia coli

PDB ID 1zeq

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