1zes

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:04, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1zes.gif|left|200px]]
 
-
{{Structure
+
==BeF3- activated PhoB receiver domain==
-
|PDB= 1zes |SIZE=350|CAPTION= <scene name='initialview01'>1zes</scene>, resolution 1.90&Aring;
+
<StructureSection load='1zes' size='340' side='right'caption='[[1zes]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=BEF:BERYLLIUM TRIFLUORIDE ION'>BEF</scene>
+
<table><tr><td colspan='2'>[[1zes]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The October 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Two-component Systems'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_10 10.2210/rcsb_pdb/mom_2015_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZES FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
|GENE= phoB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zes OCA], [https://pdbe.org/1zes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zes RCSB], [https://www.ebi.ac.uk/pdbsum/1zes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zes ProSAT]</span></td></tr>
-
 
+
</table>
-
'''BeF3- activated PhoB receiver domain'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/PHOB_ECOLI PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Response regulators (RRs), which undergo phosphorylation/dephosphorylation at aspartate residues, are highly prevalent in bacterial signal transduction. RRs typically contain an N-terminal receiver domain that regulates the activities of a C-terminal DNA binding domain in a phosphorylation-dependent manner. We present crystallography and solution NMR data for the receiver domain of Escherichia coli PhoB which show distinct 2-fold symmetric dimers in the inactive and active states. These structures, together with the previously determined structure of the C-terminal domain of PhoB bound to DNA, define the conformation of the active transcription factor and provide a model for the mechanism of activation in the OmpR/PhoB subfamily, the largest group of RRs. In the active state, the receiver domains dimerize with 2-fold rotational symmetry using their alpha4-beta5-alpha5 faces, while the effector domains bind to DNA direct repeats with tandem symmetry, implying a loss of intramolecular interactions.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/1zes_consurf.spt"</scriptWhenChecked>
-
1ZES is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZES OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states., Bachhawat P, Swapna GV, Montelione GT, Stock AM, Structure. 2005 Sep;13(9):1353-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16154092 16154092]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zes ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bachhawat, P.]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Montelione, G T.]]
+
[[Category: Two-component Systems]]
-
[[Category: Stock, A M.]]
+
[[Category: Bachhawat P]]
-
[[Category: BEF]]
+
[[Category: Montelione GT]]
-
[[Category: MG]]
+
[[Category: Stock AM]]
-
[[Category: activated]]
+
-
[[Category: chey-like fold]]
+
-
[[Category: phob]]
+
-
[[Category: response regulator]]
+
-
[[Category: transcription factor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:34:34 2008''
+

Current revision

BeF3- activated PhoB receiver domain

PDB ID 1zes

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zes"
Personal tools