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| | ==Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules== | | ==Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules== |
| - | <StructureSection load='4j0l' size='340' side='right' caption='[[4j0l]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4j0l' size='340' side='right'caption='[[4j0l]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J0L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J0L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rscc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4550 Secale cereale])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0l OCA], [https://pdbe.org/4j0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j0l RCSB], [https://www.ebi.ac.uk/pdbsum/4j0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0l ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j0l RCSB], [http://www.ebi.ac.uk/pdbsum/4j0l PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE]] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref> | + | [https://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4j0l" style="background-color:#fffaf0;"></div> |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Chitinase|Chitinase]] | + | *[[Chitinase 3D structures|Chitinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chitinase]] | + | [[Category: Large Structures]] |
| | [[Category: Secale cereale]] | | [[Category: Secale cereale]] |
| - | [[Category: Fukamizo, T]] | + | [[Category: Fukamizo T]] |
| - | [[Category: Numata, T]] | + | [[Category: Numata T]] |
| - | [[Category: Ohnuma, T]] | + | [[Category: Ohnuma T]] |
| - | [[Category: Umemoto, N]] | + | [[Category: Umemoto N]] |
| - | [[Category: Carbohydrate]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
CHIC_SECCE Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.[1] [2] [3]
Publication Abstract from PubMed
Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)(4) revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)(4) molecules. One (GlcNAc)(4) molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.
Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.,Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ohnuma T, Yagi M, Yamagami T, Taira T, Aso Y, Ishiguro M. Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c. Biosci Biotechnol Biochem. 2002 Feb;66(2):277-84. PMID:11999399
- ↑ Taira T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed. Biosci Biotechnol Biochem. 2001 Dec;65(12):2710-8. PMID:11826968
- ↑ Taira T, Ohnuma T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci Biotechnol Biochem. 2002 May;66(5):970-7. PMID:12092848
- ↑ Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710 doi:http://dx.doi.org/10.1016/j.febslet.2013.07.008
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