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4u9l

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==Structure of a membrane protein==
==Structure of a membrane protein==
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<StructureSection load='4u9l' size='340' side='right' caption='[[4u9l]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='4u9l' size='340' side='right'caption='[[4u9l]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4u9l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U9L FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4u9l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U9L FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4u9n|4u9n]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u9l RCSB], [http://www.ebi.ac.uk/pdbsum/4u9l PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u9l OCA], [https://pdbe.org/4u9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u9l RCSB], [https://www.ebi.ac.uk/pdbsum/4u9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u9l ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MGTE_THET8 MGTE_THET8]] Acts as a highly selective magnesium channel.<ref>PMID:17700703</ref> <ref>PMID:19798051</ref>
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[https://www.uniprot.org/uniprot/MGTE_THET8 MGTE_THET8] Acts as a highly selective magnesium channel.<ref>PMID:17700703</ref> <ref>PMID:19798051</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Magnesium is the most abundant divalent cation in living cells and is crucial to several biological processes. MgtE is a Mg(2+) channel distributed in all domains of life that contributes to the maintenance of cellular Mg(2+) homeostasis. Here we report the high-resolution crystal structures of the transmembrane domain of MgtE, bound to Mg(2+), Mn(2+) and Ca(2+). The high-resolution Mg(2+)-bound crystal structure clearly visualized the hydrated Mg(2+) ion within its selectivity filter. Based on those structures and biochemical analyses, we propose a cation selectivity mechanism for MgtE in which the geometry of the hydration shell of the fully hydrated Mg(2+) ion is recognized by the side-chain carboxylate groups in the selectivity filter. This is in contrast to the K(+)-selective filter of KcsA, which recognizes a dehydrated K(+) ion. Our results further revealed a cation-binding site on the periplasmic side, which regulate channel opening and prevents conduction of near-cognate cations.
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Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg(2+) channel MgtE.,Takeda H, Hattori M, Nishizawa T, Yamashita K, Shah ST, Caffrey M, Maturana AD, Ishitani R, Nureki O Nat Commun. 2014 Nov 4;5:5374. doi: 10.1038/ncomms6374. PMID:25367295<ref>PMID:25367295</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Caffrey, M]]
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[[Category: Large Structures]]
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[[Category: Hattori, M]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Ishitani, R]]
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[[Category: Caffrey M]]
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[[Category: Maturana, A D]]
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[[Category: Hattori M]]
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[[Category: Nishizawa, T]]
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[[Category: Ishitani R]]
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[[Category: Nureki, O]]
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[[Category: Maturana AD]]
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[[Category: Shah, S T.A]]
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[[Category: Nishizawa T]]
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[[Category: Takeda, H]]
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[[Category: Nureki O]]
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[[Category: Yamashita, K]]
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[[Category: Shah STA]]
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[[Category: Channel]]
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[[Category: Takeda H]]
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[[Category: Magnesium]]
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[[Category: Yamashita K]]
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[[Category: Metal transport]]
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Current revision

Structure of a membrane protein

PDB ID 4u9l

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